a Cellular and Molecular Research Center , Ahvaz Jundishapur University of Medical Sciences (AJUMS) , Ahvaz , Iran.
b Department of Anatomical Sciences , Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences (AJUMS) , Ahvaz , Iran.
J Microencapsul. 2017 Dec;34(8):732-743. doi: 10.1080/02652048.2017.1393115. Epub 2017 Nov 7.
Cell encapsulation using biodegradable material has promising results for tissue engineering. Since pressure is an effective factor on stem cell behaviour and various concentrations of alginate create different pressures on the cells, therefore our goal was to evaluate the mechanical effect of 1/2% (w/v) and 1/8% (w/v) alginate containing high guluronic acid on viability and osteogenic capacity of HUCWJ cells.
Cell viability was evaluated by MTT assay after 1, 7 and 14 days. Alkaline phosphatase activity was evaluated by alkaline phosphatase assay kit after 14 and 21 days. Alizarin red S staining was performed for calcium deposition among histological section.
MTT assay showed significant difference in the mean of viability rates between groups in day 14 (p < 0.05). Alizarin red S staining was higher in the group 1.8%. In addition, there was statistically significant higher ALP activity in the group 1.8% compared to the group 1.2%.
使用可生物降解材料对细胞进行封装在组织工程中有很好的效果。由于压力是影响干细胞行为的有效因素，并且不同浓度的藻酸盐对细胞产生不同的压力，因此我们的目标是评估含有高古洛糖醛酸的 1/2%（w/v）和 1/8%（w/v）藻酸盐对 HUCWJ 细胞活力和成骨能力的机械影响。
在第 1、7 和 14 天通过 MTT 检测法评估细胞活力。在第 14 和 21 天通过碱性磷酸酶检测试剂盒评估碱性磷酸酶活性。通过组织学切片中的茜素红 S 染色评估钙沉积。
MTT 检测法表明在第 14 天各组间的平均存活率有显著差异（p < 0.05）。1.8%组的茜素红 S 染色更高。此外，与 1.2%组相比，1.8%组的 ALP 活性有统计学意义上的显著升高。
