Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University, 101 Longmian Road, Nanjing, Jiangsu 211166, China; Department of Clinical Pharmacy, Shenzhen Hospital of Traditional Chinese Medicine, 1 Fuhua Road, Shenzhen, Guangdong 518033, China.
Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University, 101 Longmian Road, Nanjing, Jiangsu 211166, China.
Biomed Pharmacother. 2017 Dec;96:792-797. doi: 10.1016/j.biopha.2017.10.050. Epub 2017 Nov 6.
The accumulation of aggregated forms of the α-Synuclein (α-Syn) is associated with the pathogenesis of Parkinson's disease (PD), a chronic progressive neurodegenerative disorder. Extensive evidences have shown the promising effects of Ginkgo biloba consumption on motor activity after PD. However, the mechanisms underline the α-Syn-induced cell damage and whether ginkgolides exert neuroprotection against this injury are unclear. Here we showed that aggregated recombinant human α-Syn, but not α-Syn monomers, triggered cell injury in cultured human neuroblastoma cell line SH-SY5Y in an apoptosis way by using flow cytometry and western blot assay. Moreover, pre-treatment with the Ginkgolide B (GB) or Bilobalide (BB) protected SH-SY5Y cells against α-Syn-induced cell viability decreases, and reduced cell apoptosis after aggregated α-Syn stimulation. Together, we firstly find that aggregated α-Syn induced cell apoptosis and GB and BB may attenuate aggregated α-Syn-induced cell apoptosis, which gives us an insight into the novel therapy for PD in future.
α-突触核蛋白（α-Syn）聚集形式的积累与帕金森病（PD）的发病机制有关，PD 是一种慢性进行性神经退行性疾病。大量证据表明，银杏叶提取物消费对 PD 后的运动活动有积极作用。然而，其潜在机制是 α-Syn 诱导的细胞损伤，以及白果内酯是否对这种损伤具有神经保护作用尚不清楚。在这里，我们通过流式细胞术和 Western blot 分析表明，聚集的重组人 α-Syn（而不是 α-Syn 单体）以凋亡方式触发培养的人神经母细胞瘤 SH-SY5Y 细胞的损伤。此外，银杏内酯 B（GB）或白果内酯（BB）预处理可防止 SH-SY5Y 细胞因聚集的 α-Syn 刺激而导致的细胞活力下降和细胞凋亡增加。总之，我们首次发现聚集的 α-Syn 诱导细胞凋亡，GB 和 BB 可能减轻聚集的 α-Syn 诱导的细胞凋亡，这为我们未来治疗 PD 提供了新的思路。
