Nadeem Amina, Mumtaz Sadaf, Naveed Abdul Khaliq, Mansoor Qaisar, Aslam Muhammad, Ismail Muhammad
Department of Physiology, Army Medical College, National University of Medical Sciences, Islamabad.
Department of Physiology, HBS Medical and Dental College, Shaheed Zulfiqar Ali Bhutto Medical University, Islamabad.
J Coll Physicians Surg Pak. 2017 Oct;27(10):625-630.
To determine the frequencies of adiponectin (ADIPOQ) C-11377G, tumor necrosis factor-alpha (TNF-α) G-308A and TNF-αG-238Asingle nucleotide polymorphisms (SNP) and their association with serum levels in Pakistani T2DM and healthy population.
Case control study.
Military Hospital, Rawalpindi, Army Medical College and Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan, from June 2012 till 2014.
Cases (n=539) and controls (n=250) comprising of T2DM and healthy subjects, respectively, belonging to Pakistani Punjabi Rajput ethnicity were genotyped for SNPs. Serum adiponectin, TNF-α, insulin, blood sugar fasting (BSF), lipid profile, body mass index (BMI), and insulin resistance (IR) was determined and correlated with genotypes.
Serum TNF-αwas significantly higher and adiponectin was lower in T2DM than healthy controls (p < 0.003 and 0.0001, respectively, Mann-Whitney U-test). The frequency of ADIPOQ CC, GC and GG was 340 (63.1%), 167 (31%) and 32 (5.9%) in T2DM patients. ADIPOQ -11377 SNPwas not significantly associated with T2DM [OR = 1.116 (95% CI 0.811.53), p = 0.27- Fisher's exact test]. Genotypes deviated from Hardy-Weinberg equilibrium. Minor alleles of TNF-αG-308A and TNF-αG-238Awere not found in either groups.
Frequency of ADIPOQ -11377 risk allele is low and does not functionally affect the serum adiponectin levels; hence, ADIPOQ C-11377G SNPis not a risk factor for T2DM in Pakistani Punjabi Rajput patients. Moreover, TNF-αG-308A and TNF-αG-238ASNPs are not prevalent in this ethnic group.
确定巴基斯坦2型糖尿病患者和健康人群中脂联素（ADIPOQ）C-11377G、肿瘤坏死因子-α（TNF-α）G-308A和TNF-α G-238A单核苷酸多态性（SNP）的频率及其与血清水平的关联。
病例对照研究。
巴基斯坦拉瓦尔品第军事医院、陆军医学院以及伊斯兰堡生物医学与基因工程研究所，2012年6月至2014年。
分别对539例2型糖尿病患者和250例健康对照进行SNP基因分型，这些病例和对照均为巴基斯坦旁遮普族拉杰普特人。测定血清脂联素、TNF-α、胰岛素、空腹血糖（BSF）、血脂谱、体重指数（BMI）和胰岛素抵抗（IR），并将其与基因型进行关联分析。
2型糖尿病患者的血清TNF-α显著高于健康对照，脂联素则低于健康对照（分别采用曼-惠特尼U检验，p < 0.003和0.0001）。2型糖尿病患者中ADIPOQ CC、GC和GG基因型的频率分别为340（63.1%）、167（31%）和32（5.9%）。ADIPOQ -11377 SNP与2型糖尿病无显著关联[比值比（OR）= 1.116（95%可信区间0.81 - 1.53），p = 0.27 - 费舍尔精确检验]。基因型偏离哈迪-温伯格平衡。两组中均未发现TNF-α G-308A和TNF-α G-238A的次要等位基因。
ADIPOQ -11377风险等位基因的频率较低，且对血清脂联素水平无功能性影响；因此，ADIPOQ C-11377G SNP不是巴基斯坦旁遮普族拉杰普特患者2型糖尿病的危险因素。此外，TNF-α G-308A和TNF-α G-238A SNPs在该族群中并不普遍。
