Nadeem Amina, Mumtaz Sadaf, Saif Muhammad Wasif, Naveed Abdul Khaliq, Aslam Muhammad, Mansoor Qaiser, Siddiqui Arif, Ismail Muhammad
Army Medical College Rawalpindi, National University of Medical Sciences.
HBS Medical College, Shaheed Zulfiqar Ali Bhutto Medical University, Islamabad.
J Pak Med Assoc. 2018 Nov;68(11):1584-1589.
To determine the association of human resistin gene RETN C-420G single nucleotide polymorphism with type 2 diabetes mellitus in a specific ethnic population..
The controlled study was conducted from June 2012 to January 2015 at Military Hospital, Rawalpindi, Army Medical College, Rawalpindi, and the Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan. Patients with type 2 diabetes and healthy controls belonging to Pakistani Punjabi Rajput ethnic group were genotyped for human resistin gene RETNC-420G single nucleotide polymorphism. Serum resistin, serum insulin, fasting blood sugar, lipid profile, body mass index and insulin resistance was determined and correlated with genotypes. SPSS 18 was used for data analysis.
Of the 789 subjects, 539(68%) were diabetics and 250(32%) were controls. Serum resistin levels were significantly higher in diabetics than controls (p<0.05). The frequency of GG, GC and CC was 15(2.8%), 322(59.75%) and 202(37.5%) in diabtics. This single nucleotide polymorphism was associated with diabetes (p<0.02).Human resistin gene RETN C-420G single nucleotide polymorphism was not associated with serum resistin, insulin, body mass index, insulin resistance and dyslipidaemia in both groups (p<0.05 each).
Human resistin gene RETN C-420G single nucleotide polymorphism was found to be a risk factor for type 2 diabetes in Pakistani Punjabi Rajput population..
确定人类抵抗素基因RETN C-420G单核苷酸多态性与特定种族人群2型糖尿病之间的关联。
2012年6月至2015年1月在巴基斯坦拉瓦尔品第军事医院、拉瓦尔品第陆军医学院以及伊斯兰堡生物医学与基因工程研究所进行了一项对照研究。对属于巴基斯坦旁遮普拉杰普特族的2型糖尿病患者和健康对照者进行人类抵抗素基因RETN C-420G单核苷酸多态性基因分型。测定血清抵抗素、血清胰岛素、空腹血糖、血脂谱、体重指数和胰岛素抵抗，并与基因型进行相关性分析。使用SPSS 18进行数据分析。
789名受试者中，539名（68%）为糖尿病患者，250名（32%）为对照者。糖尿病患者的血清抵抗素水平显著高于对照者（p<0.05）。糖尿病患者中GG、GC和CC的频率分别为15（2.8%）、322（59.75%）和202（37.5%）。这种单核苷酸多态性与糖尿病相关（p<0.02）。两组中人类抵抗素基因RETN C-420G单核苷酸多态性与血清抵抗素、胰岛素、体重指数、胰岛素抵抗和血脂异常均无关联（各p<0.05）。
发现人类抵抗素基因RETN C-420G单核苷酸多态性是巴基斯坦旁遮普拉杰普特族人群2型糖尿病的一个危险因素。
