School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia.
School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor, Malaysia.
Int J Biol Macromol. 2018 Feb;107(Pt B):2412-2421. doi: 10.1016/j.ijbiomac.2017.10.125. Epub 2017 Oct 19.
Starch-based hydrogels are promising smart materials for biomedical and pharmaceutical applications, which offer exciting perspectives in biophysical research at molecular level. This work was intended to develop, characterize and explore the properties of hydrogel from starch extracted from new source, Dioscorea hispida Dennst. Starch-mediated hydrogels were successfully synthesized via free radical polymerization method with varying concentrations of acrylic acid (AA),N,N'-methylenebisacrylamide (MBA) and sodium hydroxide (NaOH) in aqueous system. The grafting reaction between starch and AA was examined by observing the decline in intensity peak of hydrogel FTIR spectrum at 3291cm and peak around 1600-1680cm, indicating the stretching of hydroxyl group (OH) and stretching of carbon-carbon double bond (CC) respectively. The effects of cross-linker, monomer and NaOH concentration on swelling ratio and gel content in different medium and conditions were also evaluated. The thermal stability and structural morphology of as-synthesized hydrogels were studied by thermogravimetry analysis (TGA) and scanning electron microscopy (SEM). In-vitro cytotoxicity study using small intestine cell line (FHS-74 Int) revealed that the as-formulated eco-friendly-hydrogel was free from any harmful material and safe to use for future product development.
淀粉基水凝胶是一种有前途的用于生物医学和制药应用的智能材料，它在分子水平的生物物理研究中提供了令人兴奋的前景。本工作旨在开发、表征和探索来源于新资源（盾叶薯蓣）的淀粉的水凝胶的性质。通过自由基聚合方法，在水溶液中使用不同浓度的丙烯酸 (AA)、N,N'-亚甲基双丙烯酰胺 (MBA) 和氢氧化钠 (NaOH) 成功合成了淀粉介导的水凝胶。通过观察水凝胶 FTIR 光谱中 3291cm 处的强度峰和 1600-1680cm 左右的峰的下降，可以检测到淀粉与 AA 之间的接枝反应，分别表示羟基 (OH) 的拉伸和碳-碳双键 (CC) 的拉伸。还评估了交联剂、单体和 NaOH 浓度对不同介质和条件下溶胀比和凝胶含量的影响。通过热重分析 (TGA) 和扫描电子显微镜 (SEM) 研究了合成水凝胶的热稳定性和结构形态。使用小肠细胞系 (FHS-74 Int) 进行的体外细胞毒性研究表明，所制备的环保水凝胶不含任何有害物质，可安全用于未来的产品开发。
