致病性脓肿分枝杆菌马西里亚种在卡氏棘阿米巴中存活情况。
Survival of pathogenic Mycobacterium abscessus subsp. massiliense in Acanthamoeba castellanii.
作者信息
da Silva Joas L, Nguyen Jan, Fennelly Kevin P, Zelazny Adrian M, Olivier Kenneth N
机构信息
Pulmonary Branch, Division of Intramural Research, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
Microbiology Service, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA.
出版信息
Res Microbiol. 2018 Jan;169(1):56-60. doi: 10.1016/j.resmic.2017.10.001. Epub 2017 Oct 19.
Abstract
We used an amoeba model to study the intracellular growth and cytotoxicity of clinical strains of Mycobacterium abscessus subsp. massiliense (Mabsm) isolated from 2 patients (one with cystic fibrosis, the other one with idiopathic bronchiectasis) during the early (smooth colonies) and late stage (rough colonies) of chronic pulmonary infection. Acanthamoeba castellanii were infected with Mabsm (MOI 100) and samples collected every 24 h for 72 h. Results showed Mabsm is able to survive in trophozoites and persist in cysts for at least 7 days. Late Mabsm demonstrated higher cytotoxicity toward A. castellanii when compared to early strains. A. castellanii is a useful in vitro host model to study infection of Mabsm clinical isolates.
摘要
我们使用一种变形虫模型来研究脓肿分枝杆菌马西里亚种（Mabsm）临床菌株的细胞内生长及细胞毒性，这些菌株是从2例患者（1例患有囊性纤维化，另1例患有特发性支气管扩张）慢性肺部感染的早期（光滑菌落）和晚期（粗糙菌落）分离得到的。将卡氏棘阿米巴用Mabsm（感染复数为100）感染，并每24小时采集样本，持续72小时。结果显示，Mabsm能够在滋养体中存活，并在包囊中持续存在至少7天。与早期菌株相比，晚期Mabsm对卡氏棘阿米巴表现出更高的细胞毒性。卡氏棘阿米巴是研究Mabsm临床分离株感染的一种有用的体外宿主模型。
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