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通过超高效液相色谱-串联质谱分析法鉴定和测定水稻(Oryza sativa L.)籽粒中天然存在的叶酸。

Identification and determination of naturally occurring folates in grains of rice (Oryza sativa L.) by UPLC-MS/MS analysis.

作者信息

Ashokkumar Kaliyaperumal, Sivakumar Paramasivam, Saradhadevi Muthukrishnan

机构信息

a Department of Plant Biotechnology, School of Agriculture , PRIST University , Thanjavur , India.

b Department of Plant Sciences , University of Saskatchewan , Saskatchewan , Canada.

出版信息

Nat Prod Res. 2018 Jul;32(14):1733-1737. doi: 10.1080/14786419.2017.1392957. Epub 2017 Oct 23.

DOI:10.1080/14786419.2017.1392957
PMID:29058482
Abstract

The genetic potential and biofortification of India-grown rice with bioavailable folate has not been studied yet. The objectives of this study were to determine the folates concentration in four cultivars of rice through UPLC-MS/MS. Total folate concentration in rice cultivars ranged from 11.0 to 51 μg/100 g with a mean of 26.0 μg/100 g. Among the four rice cultivars, the pigmented grain cultivar Nootripathu possesses two-fold rich sources of total folates than the other three non-pigmented grain cultivars. The average value of 100 g serving of rice grains could provide the amount of recommended daily allowance (% RDA) of dietary folates (6.5%) for adults, which ranged from 2.7-12.7%. Among the 5 individual forms of folates, 5-methyltetrahydrofolate was most abundant in rice cultivars followed by 10-Formylfolic acid and folic acid. The result of this study has been useful for biofortification of folates in rice.

摘要

印度种植的水稻中生物可利用叶酸的遗传潜力和生物强化尚未得到研究。本研究的目的是通过超高效液相色谱-串联质谱法(UPLC-MS/MS)测定四个水稻品种中的叶酸浓度。水稻品种中的总叶酸浓度在11.0至51μg/100g之间,平均为26.0μg/100g。在这四个水稻品种中,有色谷粒品种Nootripathu的总叶酸含量是其他三个无色谷粒品种的两倍。100克一份的稻谷平均含量可为成年人提供膳食叶酸推荐每日摄入量(%RDA)的6.5%,范围为2.7 - 12.7%。在5种叶酸的单独形式中,5-甲基四氢叶酸在水稻品种中含量最高,其次是10-甲酰叶酸和叶酸。本研究结果对水稻中叶酸的生物强化具有重要意义。

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