State Key Laboratory of Animal Nutrition, Institute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing 100193, China; Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Food Chem. 2021 Feb 1;337:127767. doi: 10.1016/j.foodchem.2020.127767. Epub 2020 Aug 4.
Egg yolks are a good source of folates. However, the method for analyzing the naturally occurring folates in egg yolks is complicated and time-consuming. In this study, a simplified pre-treatment method followed by validated HPLC-MS/MS was developed to determine native folates in eggs from laying hens treated with different amounts of folic acid. The modified enhanced matrix removal -lipid method to purify samples showed good performance in lipid elimination, reduction of steps and time savings. According to experimental analysis, yolks contained total folate amounts ranging from 147 to 760 μg/100 g when laying hens' diet was supplemented with folic acid from 0 to 10 mg/kg. Four folate vitamers were detected in egg yolks: 5-methyltetrahydrofolate accounted for 91-98% of total folates, whereas folic acid, 5-formyltetrahydrofolate and 10-formylfolic acid together accounted for 2-9%. Therefore, laying hens efficiently converted folic acid in feed into 5-methyltetrahydrofolate in eggs with little folic acid deposition.
蛋黄是叶酸的良好来源。然而，分析蛋黄中天然叶酸的方法复杂且耗时。本研究开发了一种简化的预处理方法，然后结合经过验证的 HPLC-MS/MS 来测定经不同剂量叶酸处理的母鸡所产鸡蛋中的天然叶酸。改良的增强基质去除-脂质法用于样品纯化，在去除脂质、减少步骤和节省时间方面表现出良好的性能。根据实验分析，当母鸡饲料中添加 0 至 10mg/kg 的叶酸时，蛋黄中的总叶酸含量范围为 147 至 760μg/100g。在蛋黄中检测到四种叶酸维生素：5-甲基四氢叶酸占总叶酸的 91-98%，而叶酸、5-甲酰四氢叶酸和 10-甲酰叶酸共占 2-9%。因此，母鸡将饲料中的叶酸有效地转化为蛋黄中的 5-甲基四氢叶酸，而很少有叶酸沉积。
