Pitarokoili Kalliopi, Ambrosius Bjoern, Gold Ralf
Department of Neurology, St. Josef Hospital, Ruhr-University Bochum, Bochum, Germany.
Curr Protoc Neurosci. 2017 Oct 23;81:9.61.1-9.61.20. doi: 10.1002/cpns.36.
In this unit, we describe in detail the most common methods used to break immunological tolerance for central myelin antigens and induce experimental autoimmune encephalomyelitis (EAE) in Lewis rats as an animal model of multiple sclerosis. The resulting disease course ranges from an acute monophasic disease to a chronic relapsing or chronic progressive course, which strongly resembles the human disease. These models enable the study of cellular and humoral autoimmunity against major antigenic epitopes of the myelin basic protein, myelin oligodendrocyte glycoprotein, or proteolipid protein. We provide an overview of common immunization protocols for induction of active and passive EAE, assessment and analysis of clinical score, preparation and purification of myelin basic protein, and derivation of neuroantigen-specific rat T cell lines. Finally, we describe the major clinical characteristics of these models. © 2017 by John Wiley & Sons, Inc.
在本单元中，我们详细描述了用于打破对中枢髓鞘抗原的免疫耐受并在Lewis大鼠中诱导实验性自身免疫性脑脊髓炎（EAE）作为多发性硬化症动物模型的最常用方法。由此产生的病程范围从急性单相疾病到慢性复发或慢性进行性病程，这与人类疾病极为相似。这些模型有助于研究针对髓鞘碱性蛋白、髓鞘少突胶质细胞糖蛋白或蛋白脂蛋白主要抗原表位的细胞和体液自身免疫。我们概述了诱导主动和被动EAE的常见免疫方案、临床评分的评估和分析、髓鞘碱性蛋白的制备和纯化以及神经抗原特异性大鼠T细胞系的衍生。最后，我们描述了这些模型的主要临床特征。© 2017约翰威立父子公司版权所有
