Frank R M, Osman M, Meyer J M, Ruch J V
J Biol Buccale. 1979 Sep;7(3):225-41.
Electron microscope autoradiography was performed after isolated labeling with 3H-glucosamine of the enamel organ or the dental papilla of Swiss mice followed by reassociated toothgerm culture for 9.30 h and 19 h. After these two time intervals in case of an enamel organ labeling, numerous silver grains were observed in the intercellular spaces of the stellate reticulum indicating a concentrating and storing role of the latter. After dental papilla labeling, an almost complete absence of radioactivity was noted at 19 h. suggesting that 3H-glucosamine is not stored by the dental papilla. A clear labeling of the basement membrane area and of the adjacent aperiodic filamentous material found on the pulpal side was only observed after enamel organ labeling with 3H-glucosamine, suggesting an epithelial origin of the glycosaminoglycans found in these structures.
用3H-葡糖胺对瑞士小鼠的釉质器官或牙乳头进行单独标记，然后将重新联合的牙胚培养9.30小时和19小时后，进行电子显微镜放射自显影。在这两个时间间隔后，如果是釉质器官标记，在星网状层的细胞间隙中观察到大量银颗粒，表明星网状层具有浓缩和储存作用。牙乳头标记后，在19小时时几乎没有放射性，这表明3H-葡糖胺不被牙乳头储存。仅在用3H-葡糖胺标记釉质器官后，才观察到基底膜区域和牙髓侧相邻的无定形丝状物质有明显标记，这表明这些结构中发现的糖胺聚糖起源于上皮。
