a Key Medical Laboratory of Obstetrics and Gynecology , The Second Affiliated Hospital, Chongqing Medical University , Chongqing , China.
Cancer Biol Ther. 2017 Dec 2;18(12):984-989. doi: 10.1080/15384047.2017.1385683. Epub 2017 Oct 23.
The aim of this study was to determine whether photodynamic therapy (PDT) alone or combined with cisplatin (DDP), can deactivate cisplatin-resistant cancer cells. Human cancer cell lines A549 and SKOV3, and chemoresistant sublines A549/DDP and SKOV3/DDP, were subjected to PDT, DDP, or PDT combined with DDP. Cell viability and apoptosis were analyzed, and then intracellular reactive oxygen species (ROS) and proteins related to apoptosis were determined. PDT caused cell death, and PDT combined with DDP led to the highest percentage of dead cells in 4 cell lines; similar results were detected in ROS; a quantification evaluation manifested that the combined effect was addition. DDP increased the percentage of apoptotic cells, and the ROS level in A549 and SKOV3 cells, which was not observed in A549/DDP and SKOV3/DDP cells. Western blot revealed an increase of caspase 3 and Bax, and a decrease of Bcl-2, demonstrating the occurrence of apoptosis. The data suggest that PDT can efficiently deactivate resistant cells and enhance the action of DDP against resistant cancer cells.
本研究旨在确定光动力疗法（PDT）单独或联合顺铂（DDP）是否可以使顺铂耐药癌细胞失活。将人癌细胞系 A549 和 SKOV3 以及化疗耐药亚系 A549/DDP 和 SKOV3/DDP 进行 PDT、DDP 或 PDT 联合 DDP 处理。分析细胞活力和细胞凋亡，并测定细胞内活性氧物种（ROS）和与凋亡相关的蛋白。PDT 导致细胞死亡，并且 PDT 联合 DDP 在 4 种细胞系中导致死亡细胞的百分比最高；在 ROS 中也检测到了相似的结果；定量评估表明联合作用是相加的。DDP 增加了凋亡细胞的百分比，以及 A549 和 SKOV3 细胞中的 ROS 水平，但在 A549/DDP 和 SKOV3/DDP 细胞中未观察到。Western blot 显示 caspase 3 和 Bax 增加，Bcl-2 减少，表明发生了凋亡。数据表明 PDT 可以有效灭活耐药细胞，并增强 DDP 对耐药癌细胞的作用。
