Naus C C, Bloom F E
Department of Anatomy, University of Western Ontario, London, Canada.
Brain Res. 1988 Sep 1;471(1):61-8. doi: 10.1016/0165-3806(88)90153-8.
The development of somatostatin (SS) neurons and fibers has been examined in the dorsolateral cortex of the mouse mutant reeler. Immunohistochemistry was performed using antisera directed primarily against SS28 or SS28(1-12). In the normal mouse at postnatal day 5 (P5), somatostatin (SS) neurons are concentrated in the ventral half of the cortex, in the developing layers V and VI. In the reeler mutant, SS neurons are scattered throughout the radial extent of the cortex, being concentrated in the dorsal half of the cortex in the polymorphic and large pyramidal cell layers. By P20, when the adult pattern of SS neuron distribution is evident in the normal mouse cortex, the distribution of similar neurons in the reeler appears inverted: immunoreactive neurons are concentrated in the dorsal half of the cortex. Immunoreactive fiber distribution follows a developmental pattern similar to that observed for SS neurons. At P5, SS fibers are most dense in layer I and V-VI of the normal cortex, while in the reeler, fibers are predominant in the polymorphic and large pyramidal cell layers. By P10, many fewer immunoreactive fibers can be detected in either normal or reeler mice than at P5. Nevertheless, while SS fibers in the normal cortex are most dense in layers I and V-VI, the reeler cortex exhibits little laminar heterogeneity in the distribution of these fibers. Thus, the SS fiber distribution appears less organized in the reeler cortex. These results suggest that whatever the nature of the genetic alteration resulting in cortical cellular developmental malposition in the reeler, SS cells and fibers, representing a completely intrinsic neocortical cellular system, behave as do all other cortical elements.
已对小鼠突变体reeler背外侧皮质中生长抑素（SS）神经元和纤维的发育进行了研究。使用主要针对SS28或SS28(1-12)的抗血清进行免疫组织化学分析。在出生后第5天（P5）的正常小鼠中，生长抑素（SS）神经元集中在皮质的腹侧半部，即发育中的V层和VI层。在reeler突变体中，SS神经元分散在皮质的整个径向范围内，集中在多形和大锥体细胞层的皮质背侧半部。到P20时，正常小鼠皮质中SS神经元分布的成年模式明显，而reeler中类似神经元的分布似乎颠倒了：免疫反应性神经元集中在皮质的背侧半部。免疫反应性纤维分布遵循与SS神经元相似的发育模式。在P5时，正常皮质的I层和V-VI层中SS纤维最密集，而在reeler中，纤维在多形和大锥体细胞层中占主导。到P10时，正常或reeler小鼠中可检测到的免疫反应性纤维比P5时少得多。然而，虽然正常皮质中的SS纤维在I层和V-VI层中最密集，但reeler皮质在这些纤维的分布上几乎没有层状异质性。因此，reeler皮质中的SS纤维分布似乎组织性较差。这些结果表明，无论导致reeler皮质细胞发育错位的基因改变的性质如何，代表完全内在新皮质细胞系统的SS细胞和纤维，其行为与所有其他皮质元素相同。
