SchÄfer Bárbara Tavares, Silveira Mariana Póvoa, Palombit Kelly, Mendes Cristina Eusébio, Watanabe Ii Sei, Miglino Maria Angélica, Castelucci Patricia
Department of Surgery, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, Brazil.
Department of Anatomy/Biomedical Sciences Institute, University of São Paulo, Brazil.
Anat Rec (Hoboken). 2018 Apr;301(4):673-685. doi: 10.1002/ar.23708. Epub 2017 Nov 11.
Duchenne-like muscular dystrophy (canine dystrophinopathy) is a hereditary degenerative disease characterized by muscle changes similar to those described for Duchenne muscular dystrophy (DMD) and by alterations in the smooth muscles of the gastrointestinal tract. Some authors have suggested that these abnormalities may be associated with intestinal motility. This study analyzed the nitrergic and cholinergic neurons and P2X7 receptor expression in the myenteric plexus of the ileum and distal colon of dogs with muscular dystrophy. Immunohistochemical techniques were used to detect nitric oxide synthase (NOS) and acetylcholine transferase (ChAT) expression and to label all HuC/D- and P2X7 receptor-immunoreactive (IR) neurons. Transmission electron microscopy and basic histology were performed for further analysis. The results showed that nitrergic neurons exhibited a Dogiel type I morphology in the ileum and distal colon. The neuronal profile results showed that there were fewer NOS-, ChAT-, and HuC/D-IR neurons in the ileum than in the distal colon in the dystrophic (DT) dogs. Additionally, there were more NOS-, ChAT- and HuC/D-IR neurons per ganglion in the distal colon than in the ileum. The P2X7 receptor-expressing neurons colocalized with nitrergic and cholinergic neurons. Transmission and light microscopy revealed collagen between the muscle fibers, between the circular and longitudinal muscle layers and within the myenteric ganglia of dogs with muscular dystrophy. These findings provide a morphological description of the myenteric neurons in the ileum and distal colon of these DT dogs and may contribute to a better understanding of the gastrointestinal disorders found in patients with DMD. Anat Rec, 301:673-685, 2018. © 2017 Wiley Periodicals, Inc.
杜兴氏样肌营养不良症（犬类肌营养不良症）是一种遗传性退行性疾病，其特征是肌肉变化类似于杜兴氏肌营养不良症（DMD）所描述的变化，并且胃肠道平滑肌也有改变。一些作者认为这些异常可能与肠道蠕动有关。本研究分析了患有肌营养不良症的犬回肠和远端结肠肌间神经丛中的含氮能和胆碱能神经元以及P2X7受体表达。采用免疫组织化学技术检测一氧化氮合酶（NOS）和乙酰胆碱转移酶（ChAT）表达，并标记所有HuC/D和P2X7受体免疫反应性（IR）神经元。进行透射电子显微镜检查和基础组织学检查以作进一步分析。结果显示，含氮能神经元在回肠和远端结肠呈现I型多极神经元形态。神经元轮廓结果显示，营养不良（DT）犬的回肠中NOS、ChAT和HuC/D-IR神经元比远端结肠中的少。此外，远端结肠中每个神经节的NOS、ChAT和HuC/D-IR神经元比回肠中的多。表达P2X7受体的神经元与含氮能和胆碱能神经元共定位。透射和光学显微镜检查显示，患有肌营养不良症的犬的肌纤维之间、环行肌层和纵行肌层之间以及肌间神经节内存在胶原蛋白。这些发现提供了这些DT犬回肠和远端结肠肌间神经元的形态学描述，可能有助于更好地理解DMD患者中发现的胃肠道疾病。《解剖学记录》，301:673 - 685，2018年。© 2017威利期刊公司。
