Kulkarni Tanmay, Slaughter Gymama
Annu Int Conf IEEE Eng Med Biol Soc. 2017 Jul;2017:5-8. doi: 10.1109/EMBC.2017.8036749.
A novel biosensing system capable of simultaneously sensing glucose and powering portable electronic devices such as a digital glucometer is described. The biosensing system consists of enzymatic glucose biofuel cell bioelectrodes functionalized with pyrolloquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BOD) at the bioanode and biocathode, respectively. A dual-stage power amplification circuit is integrated with the single biofuel cell to amplify the electrical power generated. In addition, a capacitor circuit was incorporated to serve as the transducer for sensing glucose. The open circuit voltage of the optimized biofuel cell reached 0.55 V, and the maximum power density achieved was 0.23 mW/ cm at 0.29 V. The biofuel cell exhibited a sensitivity of 0.312 mW/mM.cm with a linear dynamic range of 3 mM - 20 mM glucose. The overall self-powered glucose biosensor is capable of selectively screening against common interfering species, such as ascorbate and urate and exhibited an operational stability of over 53 days, while maintaining 90 % of its activity. These results demonstrate the system's potential to replace the current glucose monitoring devices that rely on external power supply, such as a battery.
本文描述了一种新型生物传感系统，该系统能够同时检测葡萄糖并为诸如数字血糖仪等便携式电子设备供电。该生物传感系统由酶促葡萄糖生物燃料电池生物电极组成，在生物阳极和生物阴极分别用吡咯喹啉醌葡萄糖脱氢酶（PQQ-GDH）和胆红素氧化酶（BOD）进行功能化。一个双级功率放大电路与单个生物燃料电池集成在一起，以放大产生的电能。此外，还并入了一个电容电路作为检测葡萄糖的传感器。优化后的生物燃料电池开路电压达到0.55 V，在0.29 V时实现的最大功率密度为0.23 mW/cm²。该生物燃料电池对葡萄糖的灵敏度为0.312 mW/（mM·cm²），线性动态范围为3 mM至20 mM葡萄糖。整体自供电葡萄糖生物传感器能够选择性地筛选常见干扰物质，如抗坏血酸盐和尿酸盐，并且在保持90%活性的同时，表现出超过53天的操作稳定性。这些结果证明了该系统有潜力取代目前依赖外部电源（如电池）的葡萄糖监测设备。
