Kuis Robinson, Hasan Md Qumrul, Slaughter Gymama
Annu Int Conf IEEE Eng Med Biol Soc. 2019 Jul;2019:1765-1768. doi: 10.1109/EMBC.2019.8856755.
The performance of bioelectrodes in enzymatic glucose biofuel cell is not only dependent on the enzyme immobilization schemes but it is greatly influenced by the ability of the enzyme to exhibit favorable orientation for a direct electron transfer (DET) between the enzyme and the current collector. The electrochemical investigation of chitosan and nafion-chitosan coatings on multi-walled carbon nanotubes (MWCNTs) immobilized with pyrroloquinoline quinone dependent glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BOD) at the bioanode and biocathode, respectively revealed interesting operational stability performance for the enzymatic biofuel cells. The bioelectrodes operated in DET mode and the chitosan coated biofuel cell system overall demonstrated higher power (156 μW) output. The stability of PQQ-GDH bioanodes varied based on the enzyme concentrations, wherein a concentration of 2.5 mg/ml resulted in a significant enhancement in stability and the maximum power density of 1.6 mW/cm compared to enzyme concentrations of 5 mg/ml PQQ-GDH or higher.
生物电极在酶促葡萄糖生物燃料电池中的性能不仅取决于酶的固定化方案，还极大地受酶在酶与集流体之间进行直接电子转移（DET）时呈现有利取向的能力影响。分别在生物阳极和生物阴极上对固定有吡咯喹啉醌依赖性葡萄糖脱氢酶（PQQ-GDH）和胆红素氧化酶（BOD）的多壁碳纳米管（MWCNT）上的壳聚糖和纳滤-壳聚糖涂层进行电化学研究，揭示了酶促生物燃料电池有趣的操作稳定性性能。生物电极以DET模式运行，整体上壳聚糖涂层生物燃料电池系统表现出更高的功率（156 μW）输出。PQQ-GDH生物阳极的稳定性因酶浓度而异，其中2.5 mg/ml的浓度导致稳定性显著提高，与5 mg/ml或更高浓度的PQQ-GDH相比，最大功率密度为1.6 mW/cm²。
