Bertao Ana Raquel
Annu Int Conf IEEE Eng Med Biol Soc. 2017 Jul;2017:185-188. doi: 10.1109/EMBC.2017.8036793.
Urinalysis allows obtaining information about many of the body's functions. Normally, urine is a very easily collected specimen, but in some cases, it can be an uncomfortable process. Moreover, standard methods such as microscopic examination or urine culturing represent time-consuming procedures. In this regard, this work explores the design of a device that combines disposable diapers, as a platform for sample collection, with part of the dipsticks method. Even though this is a desirable combination for a faster and comfortable approach, it is also convenient to extend the prescribed time, provided by the dipstick manufacturer, to read the results. This allows the stability and validity of the results for longer, preferably until the moment of the diaper changing, and it was also considered in the design. The desired conditions were reproduced for the reagent pads of glucose and nitrite. The results show that the RGB profile, obtained in glucose detection, varied after approximately 60 min. However, when the developed colors are visually compared with the reference colors, the results suggest that there were not significant variations. In the nitrite assay, by analyzing the RGB profile or by visual comparison with the reference colors, the color does not appear to change over time, but the results were not the expected. It seems that the proposed design is promising regarding results stability, but more experiments need to be done to check if the variations are constant for more biomarkers in the conditions provided by the device.
尿液分析有助于获取有关人体许多功能的信息。通常，尿液是一种非常容易采集的样本，但在某些情况下，采集过程可能会让人感到不适。此外，诸如显微镜检查或尿液培养等标准方法是耗时的程序。在这方面，这项工作探索了一种装置的设计，该装置将一次性尿布作为样本采集平台与部分试纸法相结合。尽管这是一种实现更快且更舒适检测方法的理想组合，但延长试纸制造商规定的读取结果的规定时间也是很方便的。这使得结果的稳定性和有效性能保持更长时间，最好是直到更换尿布之时，并且在设计中也考虑到了这一点。针对葡萄糖和亚硝酸盐的试剂垫再现了所需条件。结果表明，在葡萄糖检测中获得的RGB图谱在大约60分钟后发生了变化。然而，当将显色结果与参考颜色进行视觉比较时，结果表明没有显著差异。在亚硝酸盐检测中，通过分析RGB图谱或与参考颜色进行视觉比较，颜色似乎不会随时间变化，但结果并不理想。看来所提出的设计在结果稳定性方面很有前景，但还需要进行更多实验，以检查在该装置所提供的条件下，对于更多生物标志物而言，这些变化是否恒定。
