Annu Int Conf IEEE Eng Med Biol Soc. 2015 Aug;2015:5892-5. doi: 10.1109/EMBC.2015.7319732.
Urinary tract infections (UTI) are common infection diseases in elderly patients. The conventional method of detecting UTI involves the collection of significant urine samples from the elderly patients. However, this is a very difficult and time-consuming procedure. This paper addresses the development of a microfluidic paper-based analytical device (μPAD) to detect UTI from urine collected from adult diapers. The design and fabrication for the μPAD is shown. The fabrication process involves melting solid wax on top of filter paper using a hot plate, followed by pattern transfer using a mold with rubbed wax. To demonstrate the feasibility of the proposed method, the μPAD with deposited nitrite reagent had detected different concentrations of nitrite solutions from 0.5 ppm to 100 ppm spiked in urine samples. A calibration curve was obtained by plotting the gray scale intensity values against the various nitrite concentrations. The results showed that the proposed paper-based device holds great potential as low-cost, disposable solution to sensitively detect UTI markers in urine sampled from diapers.
尿路感染（UTI）是老年患者常见的感染性疾病。传统的检测尿路感染的方法包括从老年患者中采集大量尿液样本。然而，这是一个非常困难且耗时的过程。本文介绍了一种基于微流控纸基分析装置（μPAD）的开发，用于从成人尿布收集的尿液中检测尿路感染。展示了μPAD的设计和制造过程。制造过程包括使用热板在滤纸顶部熔化固体蜡，然后使用带有摩擦蜡的模具进行图案转移。为了证明该方法的可行性，带有沉积亚硝酸盐试剂的μPAD检测了尿液样本中添加的浓度从0.5 ppm到100 ppm的不同浓度亚硝酸盐溶液。通过将灰度强度值与各种亚硝酸盐浓度作图获得校准曲线。结果表明，所提出的纸基装置作为一种低成本、一次性的解决方案，在灵敏检测从尿布采集的尿液中的尿路感染标志物方面具有巨大潜力。
