Mao L Q, Li X B
Bratisl Lek Listy. 2017;118(9):552-556. doi: 10.4149/BLL_2017_106.
To study the mechanism of NAC to improve LPS induced acutelung lung injury.
The 40 rats were divided into 4 groups included NC group, Model group, NAC group and DXM group. The rats of Model, NAC and DXM groups were injected LPS, NAC group and DXM group were resepectively injected NAC (200 mg/kg) or DXM (70 mg/kg). Collecting the bronchoalveolar lavage fluid (BALF) from lung, and measuring the TGF-β1 concentration of BALF and lung tissue in 4 groups; After executing the rats, taken the lung tissue to observant lung pathological morphology and evaluated TGF-β1 expression of difference groups. Measuring the TLR-4 and NF-κb in 4 groups by WB assay.
Compared with Model group, The NAC and DXM groups were improved in H (et) E staining, the TGF-β1 concentration of NAC and DXM groups were significantly reduced in BALF and lung tissue (p < 0.05, respectively). TLR-4 and NF-κb proteins of NAC and DXM groups were lower than that of Model group in IHC and WB assays (p < 0.05, respectively).
NAC had effects to protect LPS induced lung injury via TLR-4/NF-κb signaling pathway (Fig. 5, Ref. 19).
研究N-乙酰半胱氨酸（NAC）改善脂多糖（LPS）诱导的急性肺损伤的机制。
将40只大鼠分为4组，即正常对照组（NC组）、模型组、NAC组和地塞米松（DXM）组。模型组、NAC组和DXM组大鼠注射LPS，NAC组和DXM组分别注射NAC（200mg/kg）或DXM（70mg/kg）。收集大鼠肺支气管肺泡灌洗液（BALF），检测4组BALF及肺组织中转化生长因子-β1（TGF-β1）浓度；处死大鼠后，取肺组织观察肺病理形态，并评估不同组TGF-β1表达情况。通过蛋白质免疫印迹法（WB法）检测4组中Toll样受体4（TLR-4）和核因子κB（NF-κb）。
与模型组相比，NAC组和DXM组苏木精-伊红（HE）染色有所改善，NAC组和DXM组BALF及肺组织中TGF-β1浓度显著降低（分别为p<0.05）。免疫组化（IHC）和WB检测中，NAC组和DXM组的TLR-4和NF-κb蛋白低于模型组（分别为p<0.05）。
NAC通过TLR-4/NF-κb信号通路对LPS诱导的肺损伤具有保护作用（图5，参考文献19）。
