Suppr超能文献

接触新烟碱类杀虫剂会诱发小鼠和兔子的胚胎毒性。

Exposure to neonicotinoid insecticides induces embryotoxicity in mice and rabbits.

作者信息

Babeľová Janka, Šefčíková Zuzana, Čikoš Štefan, Špirková Alexandra, Kovaříková Veronika, Koppel Juraj, Makarevich Alexander V, Chrenek Peter, Fabian Dušan

机构信息

Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4-6, 040 01 Košice Slovak Republic.

Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4-6, 040 01 Košice Slovak Republic.

出版信息

Toxicology. 2017 Dec 1;392:71-80. doi: 10.1016/j.tox.2017.10.011. Epub 2017 Oct 20.

Abstract

The potential toxicity of neonicotinoids (thiacloprid, acetamiprid, thiamethoxam and clothianidin) as well as related commercial products Calypso 480SC (thiacloprid mixture), Mospilan 20SP (acetamiprid mixture) and Agita 10WG (thiamethoxam mixture) on developmental capacities and quality of preimplantation embryos was evaluated. During in vitro tests, isolated 2-cell stage mice embryos were cultured in media with various concentrations of active compounds or commercial products until blastocyst formation. As found using stereomicroscopic examination, all neonicotinoids at highest (100μM) concentration negatively affected embryonic development (P<0.001). Fluorescence staining revealed that the blastocysts obtained displayed lower numbers of blastomeres and elevated incidence of cell death. Thiacloprid and acetamiprid decreased quality of blastocysts also at 10μM concentration. From the tested products only Calypso 480SC containing 10μM of thiacloprid showed harmful impact on embryo quality. In an experiment using rabbit embryos, similar negative effect of thiacloprid in vitro was recorded. In vivo testing confirmed that blastocysts collected from thiacloprid-treated mice displayed lower total cell counts than blastocysts from controls. The sensitivity of embryonic cells to neonicotinoids is in the order of thiacloprid>acetamiprid, thiomethoxam>clothianidin. Thiacloprid impairs development and quality of both mouse and rabbit preimplantation embryos, and shows embryotoxicity even at acute reference dose.

摘要

评估了新烟碱类杀虫剂(噻虫啉、啶虫脒、噻虫嗪和氯噻啉)以及相关商业产品 Calypso 480SC(噻虫啉混合物)、Mospilan 20SP(啶虫脒混合物)和 Agita 10WG(噻虫嗪混合物)对植入前胚胎发育能力和质量的潜在毒性。在体外试验中,将分离的 2 细胞期小鼠胚胎培养在含有不同浓度活性化合物或商业产品的培养基中,直至形成囊胚。通过立体显微镜检查发现,所有新烟碱类杀虫剂在最高浓度(100μM)时均对胚胎发育产生负面影响(P<0.001)。荧光染色显示,所获得的囊胚显示出较少的卵裂球数量和较高的细胞死亡率。噻虫啉和啶虫脒在 10μM 浓度时也会降低囊胚质量。在所测试的产品中,只有含有 10μM 噻虫啉的 Calypso 480SC 对胚胎质量有有害影响。在使用兔胚胎的实验中,记录到噻虫啉在体外有类似的负面影响。体内试验证实,从经噻虫啉处理的小鼠收集的囊胚显示出的总细胞数低于对照组囊胚。胚胎细胞对新烟碱类杀虫剂的敏感性顺序为噻虫啉>啶虫脒,噻虫嗪>氯噻啉。噻虫啉会损害小鼠和兔植入前胚胎的发育和质量,甚至在急性参考剂量下也表现出胚胎毒性。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验