胆酸修饰的聚乙烯亚胺共递送p53质粒DNA和bcl-2反义寡脱氧核苷酸对人癌细胞生长的协同抑制作用

Synergistic Inhibition of Human Carcinoma Cell Growth Co-Delivery of p53 Plasmid DNA and bcl-2 Antisense Oligodeoxyribonucleotide by Cholic Acid-modified Polyethylenimine.

作者信息

Weecharangsan Wanlop, Opanasopit Praneet, Niyomtham Nattisa, Yingyongnarongkul Boon-Ek, Kewsuwan Prartana, Lee Robert J

机构信息

Department of Pharmaceutical Technology, Faculty of Pharmacy, Srinakharinwirot University, Nakhonnayok, Thailand

Pharmaceutical Development of Green Innovations Group (PDGIG), Faculty of Pharmacy, Silpakorn University, Nakhonpathom, Thailand.

出版信息

Anticancer Res. 2017 Nov;37(11):6335-6340. doi: 10.21873/anticanres.12085.

DOI:10.21873/anticanres.12085

PMID:29061817

Abstract

BACKGROUND/AIM: This study investigated the co-delivery of plasmid DNA and antisense oligodeoxyribonucleotide (AS ODN) into carcinoma cells by cholic acid-modified polyethylenimine (PEI-CA).

MATERIALS AND METHODS

PEI-CA/plasmid DNA and AS ODN complexes were formulated and evaluated for delivery of plasmid DNA and AS ODN in HeLa cells. The efficiency of co-delivery of plasmid DNA and AS ODN was evaluated by cell growth inhibition using p53 and bcl-2 AS ODN.

RESULTS

AS ODN intracellular delivery and green fluorescent protein expression upon cellular transfection were greater than in cells treated with uncomplexed nucleic acids. Treatment of the cells with PEI-CA/p53 plasmid DNA and bcl-2 AS ODN complexes resulted in cell growth inhibition that was greater than that of either PEI-CA/p53 plasmid DNA complexes or PEI-CA/bcl-2 AS ODN complexes alone.