Müller Leonie, Radtke Arlo, Decker Jennifer, Koch Michael, Belge Gazanfer
Faculty of Biology and Chemistry, University of Bremen, Bremen, Germany.
Department of Neuropharmacology - Brain Research Institute, University of Bremen, Bremen, Germany.
Anticancer Res. 2017 Nov;37(11):6341-6345. doi: 10.21873/anticanres.12086.
Studies have revealed that cancer might be treated with cannabinoids since they can influence cancer cell survival. These findings suggest an alternative treatment option to chemo- and radiotherapy, that are associated with numerous adverse side-effects for the patients.
Viability staining was conducted on lung cancer, testicular cancer and neuroblastoma cells treated with different concentrations of the synthetic cannabinoid WIN 55,212-2 and the percentage of dead cells was compared. Activity of apoptosis-related enzymes was investigated by the presence of DNA ladder in gel electrophoresis.
Treatment with different WIN 55,212-2 concentrations led to a significant dose-dependent reduction of cell viability. A DNA ladder was observed after WIN 55,212-2 treatment of testicular cancer and lung cancer cells.
The application of WIN 55,212-2 was found to trigger cell death in the investigated cell lines. The decline in lung cancer and testicular cancer cell viability seems to have been caused by apoptosis. These findings may contribute to development of alternative cancer therapy strategies.
研究表明，大麻素可能对癌症具有治疗作用，因为它们能够影响癌细胞的存活。这些发现提示了一种替代化疗和放疗的治疗选择，化疗和放疗会给患者带来诸多不良副作用。
对用不同浓度的合成大麻素WIN 55,212-2处理的肺癌、睾丸癌和神经母细胞瘤细胞进行活力染色，并比较死细胞的百分比。通过凝胶电泳中DNA梯带的出现来研究凋亡相关酶的活性。
用不同浓度的WIN 55,212-2处理导致细胞活力显著呈剂量依赖性降低。在用WIN 55,212-2处理睾丸癌细胞和肺癌细胞后观察到了DNA梯带。
发现应用WIN 55,212-2可在研究的细胞系中引发细胞死亡。肺癌和睾丸癌细胞活力的下降似乎是由凋亡引起的。这些发现可能有助于开发替代性癌症治疗策略。
