DR4型巨细胞动脉炎患者的人类白细胞抗原II类基因多态性
HLA class II genes polymorphism in DR4 giant cell arteritis patients.
作者信息
Bignon J D, Ferec C, Barrier J, Pennec Y, Verlingue C, Cheneau M L, Lucas V, Muller J Y, Saleun J P
机构信息
Laboratoire d'histocompatibilité, Centre Régional de Transfusion Sanguine, Nantes, France.
出版信息
Tissue Antigens. 1988 Nov;32(5):254-8. doi: 10.1111/j.1399-0039.1988.tb01664.x.
We have previously reported a significant increase of HLA-DR4 antigen frequency in giant cell arteritis (GCA). This finding suggested an important role of immunogenetic factors in this syndrome. Recent data suggest that inherited susceptibility to several autoimmune diseases was associated with specific DR4 associated DQ beta alleles. DNAs from 27 DR4 positive patients with GCA were digested with Taq I and Bam HI, analysed on 0.7% agarose gel and hybridized with DR beta, DQ alpha and DQ beta probes. DR beta hybridization produced no variant detectable within DR4. DQ beta probe confirmed two clusters among DR4 associated DQW3 alleles: DQW 3.1 (Bam HI 360 Kb) and DQw 3.2 (Taq I 1.9 Kb and Bam HI 11 Kb). Among our 27 DR4 positive patients, 34% were DQW 3.1 and 66% were DQW 3.2. These frequencies are the same as those observed in healthy controls.
我们之前报道过巨细胞动脉炎（GCA）中HLA - DR4抗原频率显著增加。这一发现提示免疫遗传因素在该综合征中起重要作用。近期数据表明，几种自身免疫性疾病的遗传易感性与特定的DR4相关DQβ等位基因有关。对27例DR4阳性的GCA患者的DNA用Taq I和Bam HI进行消化，在0.7%琼脂糖凝胶上进行分析，并用DRβ、DQα和DQβ探针进行杂交。DRβ杂交在DR4内未检测到可变异体。DQβ探针证实了DR4相关DQW3等位基因中的两个簇：DQW 3.1（Bam HI 360 Kb）和DQw 3.2（Taq I 1.9 Kb和Bam HI 11 Kb）。在我们的27例DR4阳性患者中，34%为DQW 3.1，66%为DQW 3.2。这些频率与健康对照中观察到的频率相同。
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