Ortmann M, Vierbuchen M, Koller G, Fischer R
Institute of Pathology, University of Cologne, Köln, Federal Republic of Germany.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1988;56(3):165-73.
Using a polyclonal antibody raised against bovine heart cytochrome c oxidase, the occurrence of this mitochondrial marker enzyme has been investigated in 63 kidney tumors (ten renal oncocytomas, 43 renal cell carcinomas and ten tubulopapillary adenomas) as well as in normal renal tissue by an immunoperoxidase method (PAP-technique). The differentiation between renal oncocytomas and mitochondria-rich carcinomas represents a problem of histopathology since these tumors have a different prognosis and require different patient managements. The strong immunoreactivity in renal oncocytomas contrasted with the much weaker reactivity in renal carcinomas and adenomas. Even mitochondria-rich (granular cell type) carcinomas exhibited only moderate staining intensity. Furthermore, single strongly stained oncocytes or small complexes were sometimes detected in normal renal tissue. The demonstration of marked differences in enzyme content between renal oncocytomas and granular cell carcinomas renders this method suitable for unequivocal distinction between these renal neoplasms. The antibody proved to be a valuable marker for detecting "true" oncocytic transformation in renal tumors and was useful in defining even single oncocytes or small oncocytic lesions.
利用针对牛心脏细胞色素c氧化酶产生的多克隆抗体，通过免疫过氧化物酶法（PAP技术），在63例肾肿瘤（10例肾嗜酸细胞瘤、43例肾细胞癌和10例管状乳头状腺瘤）以及正常肾组织中研究了这种线粒体标记酶的存在情况。肾嗜酸细胞瘤与富含线粒体的癌之间的鉴别是组织病理学中的一个问题，因为这些肿瘤预后不同，需要不同的患者管理方式。肾嗜酸细胞瘤中的强免疫反应性与肾癌和腺瘤中弱得多的反应性形成对比。即使是富含线粒体的（颗粒细胞型）癌也仅表现出中等染色强度。此外，在正常肾组织中有时会检测到单个强染色的嗜酸细胞或小复合体。肾嗜酸细胞瘤和颗粒细胞癌之间酶含量的显著差异表明，该方法适用于明确区分这些肾肿瘤。该抗体被证明是检测肾肿瘤中“真正”嗜酸细胞转化的有价值标记物，甚至对于确定单个嗜酸细胞或小的嗜酸细胞病变也很有用。
