McInnes Elizabeth, Bennett Mark, O'Hara Mandy, Rasmussen Lorna, Fung Peony, Nicholls Philip, Slaven Michael, Stevenson Robert
Cerberus Sciences, Unit 3, 49 Holland Street, Thebarton, SA 5031, Australia.
School of Veterinary and Life Sciences, Murdoch University, South Street, Murdoch, WA 6150, Australia.
Vet Sci. 2015 Jun 11;2(2):84-96. doi: 10.3390/vetsci2020084.
The kidneys from six immunodeficient mice examined by Cerberus Sciences and the Animal Resources Centre, displayed karyomegaly with pale eosinophilic, intranuclear inclusions upon histopathological examination. Electron microscopy performed on kidney tissue from 5/6 mice demonstrated margination of the chromatin in large nuclei. Laboratory tests were used to detect nucleic acid of papillomaviruses, polyomaviruses, circoviruses and anelloviruses (4/6 mice), a specific PCR was used to detect murine polyomavirus (1/6), and a panel of serological tests was used to detect seroconversion to major murine pathogens (1/6). All molecular and serological tests were negative. Immunohistochemistry using polyclonal anti-bovine papillomavirus type 1 (BPV-1) L1 antibody, Camvir monoclonal anti-papillomavirus antibody (directed against the seven amino acids GFGAMDF found in human papillomavirus (HPV) 16 L1 protein), a commercially available mixture of two monoclonal antibodies, anti-BPV-1 L1/1H8 + Camvir antibodies, and a monoclonal anti-Hsc70 antibody revealed specific, positive staining of murine renal tubular epithelial intranuclear inclusions in 6/6 mice using the anti-BPV-1 L1 containing antibodies only. Methyl pyronin green, PAS and Feulgen histochemical reactions revealed that the intranuclear inclusions did not consist of RNA, DNA or carbohydrate. An immunohistochemical method now exists that can be used to confirm and evaluate suspected cases of murine inclusion body nephropathy.
由刻耳柏洛斯科学公司和动物资源中心检查的六只免疫缺陷小鼠的肾脏，在组织病理学检查时显示出核肿大，伴有淡嗜酸性核内包涵体。对6只小鼠中的5只肾脏组织进行电子显微镜检查，结果显示大细胞核中染色质边缘化。实验室检测用于检测乳头瘤病毒、多瘤病毒、圆环病毒和环病毒的核酸（6只小鼠中的4只），使用特异性聚合酶链反应检测小鼠多瘤病毒（6只小鼠中的1只），并使用一组血清学检测来检测对主要小鼠病原体的血清转化（6只小鼠中的1只）。所有分子和血清学检测均为阴性。使用多克隆抗1型牛乳头瘤病毒（BPV-1）L1抗体、Camvir单克隆抗乳头瘤病毒抗体（针对人乳头瘤病毒（HPV）16 L1蛋白中发现的七个氨基酸GFGAMDF）、两种单克隆抗体的市售混合物抗BPV-1 L1/1H8 + Camvir抗体以及单克隆抗Hsc70抗体进行免疫组织化学检测，结果显示仅使用含抗BPV-1 L1抗体时，6只小鼠的小鼠肾小管上皮核内包涵体均有特异性阳性染色。甲基派洛宁绿、过碘酸希夫反应和福尔根组织化学反应显示核内包涵体不包含RNA、DNA或碳水化合物。现在有一种免疫组织化学方法可用于确诊和评估疑似小鼠包涵体肾病病例。
