Chen Alice Che-Ha, Keleher Annie, Kedda Mary-Anne, Spurdle Amanda B, McMillan Nigel A J, Antonsson Annika
The University of Queensland, Diamantina Institute for Cancer, Immunology and Metabolic Medicine, Princess Alexandra Hospital, Woolloongabba, Brisbane, QLD, Australia.
J Med Virol. 2009 Oct;81(10):1792-6. doi: 10.1002/jmv.21592.
Recent studies have shown that human papillomavirus (HPV) DNA can be found in circulating blood, including peripheral blood mononuclear cells (PBMCs), sera, plasma, and arterial cord blood. In light of these findings, DNA extracted from PBMCs from healthy blood donors were examined in order to determine how common HPV DNA is in blood of healthy individuals. Blood samples were collected from 180 healthy male blood donors (18-76 years old) through the Australian Red Cross Blood Services. Genomic DNA was extracted and specimens were tested for HPV DNA by PCR using a broad range primer pair. Positive samples were HPV-type determined by cloning and sequencing. HPV DNA was found in 8.3% (15/180) of the blood donors. A wide variety of different HPV types were isolated from the PBMCs; belonging to the cutaneous beta and gamma papillomavirus genera and mucosal alpha papillomaviruses. High-risk HPV types that are linked to cancer development were detected in 1.7% (3/180) of the PBMCs. Blood was also collected from a healthy HPV-positive 44-year-old male on four different occasions in order to determine which blood cell fractions harbor HPV. PBMCs treated with trypsin were negative for HPV, while non-trypsinized PBMCs were HPV-positive. This suggests that the HPV in blood is attached to the outside of blood cells via a protein-containing moiety. HPV was also isolated in the B cells, dendritic cells, NK cells, and neutrophils. To conclude, HPV present in PBMCs could represent a reservoir of virus and a potential new route of transmission.
近期研究表明，在循环血液中可检测到人类乳头瘤病毒（HPV）DNA，包括外周血单核细胞（PBMC）、血清、血浆和脐带血。鉴于这些发现，对从健康献血者的PBMC中提取的DNA进行检测，以确定HPV DNA在健康个体血液中的普遍程度。通过澳大利亚红十字会血液服务机构，从180名健康男性献血者（年龄在18 - 76岁）采集血样。提取基因组DNA，并使用广谱引物对通过PCR检测标本中的HPV DNA。对阳性样本通过克隆和测序确定HPV类型。在8.3%（15/180）的献血者血液中发现了HPV DNA。从PBMC中分离出多种不同类型的HPV；属于皮肤β和γ乳头瘤病毒属以及黏膜α乳头瘤病毒。在1.7%（3/180）的PBMC中检测到与癌症发生相关的高危HPV类型。还在4个不同时间从一名44岁HPV阳性健康男性采集血液，以确定哪些血细胞组分含有HPV。用胰蛋白酶处理的PBMC对HPV呈阴性，而未用胰蛋白酶处理的PBMC呈HPV阳性。这表明血液中的HPV通过含蛋白质部分附着在血细胞外部。在B细胞、树突状细胞、NK细胞和中性粒细胞中也分离出了HPV。总之，PBMC中存在的HPV可能代表病毒库和潜在的新传播途径。
