Ezemaduka Anastasia N, Wang Yunbiao, Li Xiujun
Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130012, China.
J Nematol. 2017 Sep;49(3):334-340.
Small heat shock proteins (sHSP) are ubiquitously found in all organisms, and with other heat shock proteins (HSP) such as HSP60, HSP70, HSP90, HSP100 made up the molecular chaperone family. They are involved in a wide range of biological processes which include among others cell resistance to biological and environmental stress conditions. In this study, we show by western blotting that CeHSP17, an sHSP of , is significantly induced by high temperatures. Furthermore, in response to metal stress, the CeHSP17 protein expression was significantly induced by cadmium and zinc at high concentration of clearly cytotoxic range in wild-type . Altogether, our results show the involvement of CeHSP17 protein in both environmental and biological stresses in and establish for the first time the expression pattern of the CeHSP17 protein in response to thermal and metal stress conditions in . The responses of CeHSP17 protein expression may serve as potential sensitive biomarker for metal-induced toxicity monitoring and environmental risk assessment.
小分子热休克蛋白(sHSP)普遍存在于所有生物体中,并且与其他热休克蛋白(HSP)如HSP60、HSP70、HSP90、HSP100共同构成分子伴侣家族。它们参与广泛的生物学过程,其中包括细胞对生物和环境应激条件的抗性。在本研究中,我们通过蛋白质免疫印迹法表明,秀丽隐杆线虫的sHSP——CeHSP17在高温下被显著诱导。此外,在金属应激反应中,在野生型秀丽隐杆线虫中,高浓度(明显处于细胞毒性范围)的镉和锌显著诱导了CeHSP17蛋白的表达。总之,我们的结果表明CeHSP17蛋白参与了秀丽隐杆线虫的环境应激和生物应激,并首次确立了CeHSP17蛋白在秀丽隐杆线虫中对热应激和金属应激条件的表达模式。CeHSP17蛋白表达的反应可能作为金属诱导毒性监测和环境风险评估的潜在敏感生物标志物。