Museum and Institute of Zoology, Polish Academy of Sciences, Twarda 51/55, 00-818 Warsaw, Poland.
Department of Entomology and Animal Pests, Institute of Plant Protection-National Research Institute, Węgorka 20, 60-318 Poznan, Poland.
Int J Mol Sci. 2024 Apr 29;25(9):4867. doi: 10.3390/ijms25094867.
is one of the most important nematode pathogens. It is a sedentary, biotrophic parasite of plants that overwinters in the soil or in diseased roots. The development of is temperature dependent. Numerous studies have been performed on the effect of temperature on the development of , but only a few of them analyzed the heat shock protein () genes. The aim of the study was to perform expression profiling of eight genes (90, 1, 4, 6, 60, 19, 43, and 12.2) at two development stages of , i.e., in eggs and second-stage juveniles (J2). The eggs and J2 were incubated under cold stress (5 °C), heat stress (35 °C, 40 °C), and non-stress (10 °C, 20 °C, and 30 °C) conditions. Expression profiling was performed by qPCR. It was demonstrated that only two genes, 60 and 19, have been upregulated by heat and cold stress at both development stages. Heat stress upregulated the expression of more genes than cold stress did. The level of upregulation of most genes was more marked in J2 than in eggs. The obtained results suggest that the 90 and 1 genes can be used as bioindicators of environmental impacts on nematodes of the genus.
是最重要的线虫病原体之一。它是一种定居的、植物专性生物营养寄生虫，在土壤或患病的根部中越冬。的发育受温度的影响。已经有许多关于温度对发育影响的研究，但其中只有少数分析了热休克蛋白()基因。本研究的目的是在发育的两个阶段（卵和第二阶段幼虫（J2））对 8 个 基因（90、1、4、6、60、19、43 和 12.2）进行表达谱分析。将卵和 J2 在冷应激（5°C）、热应激（35°C、40°C）和非应激（10°C、20°C 和 30°C）条件下孵育。通过 qPCR 进行表达谱分析。结果表明，只有两个基因，60 和 19，在两个发育阶段都被热和冷应激上调。热应激上调的基因数量多于冷应激。大多数基因的上调水平在 J2 中比在卵中更为显著。研究结果表明，90 和 1 基因可以作为环境对属线虫影响的生物标志物。
