Ramani Ramya, Ramachandran Abirami, Ravindran Sriram, Kurian Gino A
School of Chemical and Biotechnology, SASTRA University, Thanjavur, India.
Vascular Biology Lab, SASTRA University, Thanjavur, 613401 India.
Indian J Clin Biochem. 2017 Oct;32(4):487-492. doi: 10.1007/s12291-016-0625-2. Epub 2016 Nov 17.
Sodium thiosulfate (STS), a cyanide antidote has been reported to possess antioxidant and calcium chelation effects, useful for the treatment of renal failure due to vascular calcification and urolithiasis. The present study investigated the in vivo modulatory effects of STS on erythrocyte calcium, phosphorous levels, lipid peroxidation, antioxidant enzyme and membrane ATPase activities (Ca, NaK, Mg and 5'' nucleotidase) in an adenine induced model of vascular calcification in rats. Adenine (0.75%) was supplemented through the diet for 28 days, which resulted in significantly ( < 0.05) increased circulating calcium and phosphorous product and oxidative stress within the RBCs, as measured from lipid peroxidation and reduced antioxidant enzymes. The membrane ATPase activities were altered (increased Ca, NaK ATPase and decreased Mg ATPase, 5' nucleotidase) compared to the rats fed on normal diet. STS (400 mg/kg) given orally was effective in establishing a normalcy in the RBC alterations. This effect was more pronounced, when STS was given from day 28 to day 49 after induction of calcification, instead of day 0 to day 28. These findings may benefit to evaluate the effectiveness of STS therapy in patients with chronic renal failure associated with increased circulating calcium and phosphorous product that leads to stiffening of vascular smooth muscles of aorta, due to calcium deposition.
硫代硫酸钠（STS）作为一种氰化物解毒剂，据报道具有抗氧化和钙螯合作用，可用于治疗因血管钙化和尿路结石引起的肾衰竭。本研究在腺嘌呤诱导的大鼠血管钙化模型中，研究了STS对红细胞钙、磷水平、脂质过氧化、抗氧化酶和膜ATP酶活性（钙、钠钾、镁和5'核苷酸酶）的体内调节作用。通过饮食补充腺嘌呤（0.75%），持续28天，这导致循环钙和磷产物显著增加（P<0.05），红细胞内氧化应激增加，这可通过脂质过氧化和抗氧化酶减少来衡量。与正常饮食喂养的大鼠相比，膜ATP酶活性发生了改变（钙、钠钾ATP酶增加，镁ATP酶、5'核苷酸酶减少）。口服给予STS（400mg/kg）可有效使红细胞改变恢复正常。当在钙化诱导后第28天至第49天给予STS，而不是从第0天至第28天给予时，这种效果更为明显。这些发现可能有助于评估STS疗法对患有慢性肾衰竭且循环钙和磷产物增加导致主动脉血管平滑肌因钙沉积而僵硬的患者的有效性。
