产生抗人干扰素单克隆抗体的杂交瘤的制备
Preparation of Hybridomas producing monoclonal antibodies against human interferon.
作者信息
Andzhaparidze O G, Nagieva F G, Pille E R, Nikulina V G, Barkova E P, Etkind G V, Melnikova N L, Sidorova E V
机构信息
Moscow Research Institute of Viral Preparations, U.S.S.R. Ministry of Health.
出版信息
Acta Virol. 1988 Nov;32(6):481-6.
To prepare hybridomas secreting monoclonal antibodies (MoAb) against human alpha-interferon (alpha-IFN), BALB/c mice were immunized with IFN produced in Namalwa cells. Native alpha-IFN, as well as partially purified or on cellulose adsorbed alpha-IFN preparations were used for immunization. Seven hybridomas continuously secreting IgG against human alpha-IFN were prepared by fusion of splenocytes from immunized donors with the mouse myeloma cells. MoAb reacted in ELISA as well as in neutralization test with human lymphoblastoid, leukocytic and recombinant alpha-IFN.
为制备分泌抗人α-干扰素（α-IFN）单克隆抗体（MoAb）的杂交瘤，用在Namalwa细胞中产生的干扰素免疫BALB/c小鼠。天然α-干扰素以及部分纯化的或吸附在纤维素上的α-干扰素制剂用于免疫。通过将免疫供体的脾细胞与小鼠骨髓瘤细胞融合，制备了7个持续分泌抗人α-干扰素IgG的杂交瘤。单克隆抗体在ELISA以及中和试验中与人淋巴母细胞、白细胞和重组α-干扰素发生反应。
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