Ratih Dewi, Handharyani Ekowati, Setiyaningsih Surachmi
Laboratory of Pathology, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Indonesia.
Department of Clinic Reproduction and Pathology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia.
Vet World. 2017 Sep;10(9):1066-1071. doi: 10.14202/vetworld.2017.1066-1071. Epub 2017 Sep 13.
The aim of the study was to examine pathology and the distribution pattern of Newcastle disease virus (NDV) in internal organs of chickens from a field case using immunohistochemical staining.
10 groups of broiler, layer, and domestic chicken were collected from necropsy room Division of Pathology, Bogor Agricultural University. These chickens were originated from West Java and collected based on pathologist diagnosis as suspect of Newcastle disease (ND). They were subsequently confirmed positive of ND with real-time-reverse transcription polymerase chain reaction assay. The respiratory, circulatory, digestive, lymphoreticular and central nervous systems were collected for histopathology examination.
The gross pathology and histopathology changes were tracheitis, pneumonia, pericarditis, myocarditis, catarrhal proventriculitis, catarrhal enteritis, typhlitis, perihepatitis, pancreatitis, nephritis interstitial, splenitis, atrophy of Bursa Fabricius, and encephalitis.
The distribution pattern of NDV in internal organs of chickens from a field case in this study is similar with a previous reported pattern in systemic cases of the internal chicken organs. High intensity of immunohistochemistry stain result was detected in trachea, lung, proventriculus, duodenum, cecal tonsil, kidney, and brain.
本研究旨在通过免疫组织化学染色检查来自一例实际病例的鸡内脏中新城疫病毒（NDV）的病理学及分布模式。
从茂物农业大学病理学系尸体剖检室收集了10组肉鸡、蛋鸡和家鸡。这些鸡来自西爪哇，根据病理学家的诊断作为新城疫（ND）疑似病例收集。随后通过实时逆转录聚合酶链反应检测确认它们为ND阳性。收集呼吸、循环、消化、淋巴网状和中枢神经系统用于组织病理学检查。
大体病理学和组织病理学变化为气管炎、肺炎、心包炎、心肌炎、卡他性腺胃炎、卡他性肠炎、盲肠炎、肝周炎、胰腺炎、间质性肾炎、脾炎、法氏囊萎缩和脑炎。
本研究中实际病例鸡内脏中NDV的分布模式与先前报道的鸡内脏系统病例模式相似。在气管、肺、腺胃、十二指肠、盲肠扁桃体、肾脏和脑中检测到高强度的免疫组织化学染色结果。
