Thong-Asa Wachiryah, Tumkiratiwong Panas, Bullangpoti Vasakorn, Kongnirundonsuk Kasem, Tilokskulchai Kanokwan
Physiology Division, Animal Toxicology and Physiology Specialty Research Unit (ATPRU), Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Animal Toxicology and Physiology Specialty Research Unit (ATPRU), Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Avicenna J Phytomed. 2017 Sep-Oct;7(5):389-400.
The present study investigated the effects of a local Thai vegetable (Colebr.) Diels, also known as Yanang, against cerebral ischemia/reperfusion injury in mice.
Thirty male ICR mice were divided into three experimental groups of BLCCAO + 10% Tween 80, BLCCAO + 300 mg/kg, and BLCCAO + 600 mg/kg. Cerebral ischemia/reperfusion was induced by three minutes of bilateral common carotid artery occlusion (BLCCAO) followed by 18 days of reperfusion. Leaf extract was administered orally 24 hours after arterial occlusion and continued for 18 consecutive days. Cognitive abilities were evaluated using the Morris water maze. Histological analysis was conducted in the dorsal hippocampus subregions CA1, CA3, and DG and white matter regions (the corpus callosum, internal capsule, and optic tract) using 0.1 % cresyl violet and 0.1% Luxol fast blue staining.
Results showed that leaf extract at the doses of 300 and 600 mg/kg significantly enhanced spatial learning, and learning flexibility, and prevented neuronal death in the DG of mice following ischemia/reperfusion.
leaf extract enhanced spatial learning, and learning flexibility, and prevented DG neuronal death in a mice model of cerebral ischemia/reperfusion.
本研究调查了一种当地泰国蔬菜（科尔布鲁克氏豆）迪尔斯（也称为亚南）对小鼠脑缺血/再灌注损伤的影响。
将30只雄性ICR小鼠分为三个实验组，分别为双侧颈总动脉闭塞（BLCCAO）+10%吐温80组、BLCCAO+300mg/kg组和BLCCAO+600mg/kg组。通过双侧颈总动脉闭塞（BLCCAO）3分钟，然后再灌注18天诱导脑缺血/再灌注。在动脉闭塞24小时后口服叶提取物，并连续服用18天。使用莫里斯水迷宫评估认知能力。使用0.1%甲酚紫和0.1%卢戈氏坚牢蓝染色，对背侧海马体CA1、CA3和齿状回亚区域以及白质区域（胼胝体、内囊和视束）进行组织学分析。
结果表明，300mg/kg和600mg/kg剂量的叶提取物显著增强了小鼠缺血/再灌注后的空间学习能力、学习灵活性，并防止了齿状回神经元死亡。
叶提取物增强了脑缺血/再灌注小鼠模型的空间学习能力、学习灵活性，并防止了齿状回神经元死亡。
