Thong-Asa Wachiryah, Bullangpoti Vasakorn
Animal Toxicology and Physiology Specialty Research Unit (ATPSRU), Physiology Division, Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, Thailand.
Avicenna J Phytomed. 2020 Mar-Apr;10(2):202-212.
The present study investigated possible neuroprotective effects of ethanolic extract of leaf against cerebral ischemic-reperfusion injury in mice.
Forty male Institute of Cancer Research (ICR) mice were randomly divided into five groups: (1) Sham + 10% Tween 80, (2) bilateral common carotid artery occlusion (BCCAO) + 10% Tween 80, (3) BCCAO + 300 mg/kg, (4) BCCAO + 600 mg/kg and (5) BCCAO + quercetin 10 mg/kg. Cerebral ischemic-reperfusion (IR) was induced by 30 min of BCCAO followed by 45 min of reperfusion. After IR induction, total brain protein, calcium, malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), and reduced glutathione (GSH), as well as brain infraction and histopathological changes in vulnerable brain areas, such as the cerebral cortex and hippocampus, were evaluated.
The results showed that 2 weeks of pretreatment with leaf extract at doses of 300 and 600 mg/kg significantly reduced calcium and MDA, but increased GSH and SOD and CAT activities. The extract significantly attenuated brain infarction and neuronal death in the cerebral cortex and hippocampus.
We demonstrated the neuroprotective effects of leaf extract against cerebral IR injury in mice.
本研究调查了叶乙醇提取物对小鼠脑缺血再灌注损伤可能的神经保护作用。
40只雄性癌症研究所（ICR）小鼠随机分为五组：（1）假手术+10%吐温80；（2）双侧颈总动脉闭塞（BCCAO）+10%吐温80；（3）BCCAO+300毫克/千克；（4）BCCAO+600毫克/千克；（5）BCCAO+槲皮素10毫克/千克。通过30分钟的BCCAO诱导脑缺血再灌注（IR），随后再灌注45分钟。IR诱导后，评估全脑蛋白质、钙、丙二醛（MDA）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和还原型谷胱甘肽（GSH），以及脑梗死和易损脑区（如大脑皮层和海马体）的组织病理学变化。
结果表明，以300和600毫克/千克剂量的叶提取物预处理2周可显著降低钙和MDA，但增加GSH以及SOD和CAT活性。该提取物显著减轻了大脑皮层和海马体的脑梗死和神经元死亡。
我们证明了叶提取物对小鼠脑IR损伤具有神经保护作用。
