Arenas Elizabeth Hashim, Trinidad Trinidad Palad
Department of Food Technology, College of Education, University of Santo Tomas, Espana, Manila, 1015, Philippines.
The Graduate School, University of Santo Tomas, Espana, Manila, 1015, Philippines.
Avicenna J Phytomed. 2017 Sep-Oct;7(5):467-476.
Pili ( Engl) pomace is an underutilized agricultural waste that possesses great potential to be regarded as a functional food ingredient. The aim of this study was to measure the polyphenol content and antioxidant activity of pili pomace drink and determine the influence of heating on these parameters. Moreover, it sought to assess the acute effects of thermally processed pili pomace drink on plasma antioxidant and polyphenol status in humans.
Ten healthy adults received a single dose (130 ml) of pili pomace drink following an overnight fasting, and blood was collected at 0, 30, 60, 120 and 240 min after ingestion of pili pomace. Plasma total polyphenol content was measured using Folin-Ciocalteu method, while total antioxidant capacity (TAC) was determined using ferric reducing antioxidant power (FRAP) assay in uricase-treated and untreated plasma samples.
Significant changes in plasma antioxidant and polyphenol levels were observed, reaching maximum levels at 120 and between 30 - 60 min, respectively (p<0.05). Both plasma polyphenols and TAC remained significantly above baseline values throughout the entire test period (p<0.05).
Results raised the possibility that an acute consumption of this phenolic-rich pili pomace drink may enhance plasma antioxidant and polyphenol status in humans. Future studies on other unidentified metabolites from pili pomace that may have enhanced the antioxidant activity of plasma should be done.
菲律宾油梨果渣是一种未得到充分利用的农业废弃物,具有被视为功能性食品成分的巨大潜力。本研究的目的是测定菲律宾油梨果渣饮料中的多酚含量和抗氧化活性,并确定加热对这些参数的影响。此外,它还试图评估经热处理的菲律宾油梨果渣饮料对人体血浆抗氧化剂和多酚状态的急性影响。
10名健康成年人在空腹过夜后接受单剂量(130毫升)的菲律宾油梨果渣饮料,并在摄入菲律宾油梨果渣后的0、30、60、120和240分钟采集血液。血浆总多酚含量采用福林-酚试剂法测定,而总抗氧化能力(TAC)则在经尿酸酶处理和未经处理的血浆样本中使用铁还原抗氧化能力(FRAP)测定法进行测定。
观察到血浆抗氧化剂和多酚水平有显著变化,分别在120分钟和30 - 60分钟之间达到最高水平(p<0.05)。在整个测试期间,血浆多酚和TAC均显著高于基线值(p<0.05)。
结果表明,急性饮用这种富含酚类的菲律宾油梨果渣饮料可能会提高人体血浆抗氧化剂和多酚状态。未来应针对菲律宾油梨果渣中其他可能增强血浆抗氧化活性的未知代谢物开展研究。