Xu Wei, Liao Yalin
School of Veterinary and Life Sciences, Murdoch University, Murdoch, WA, 6150, Australia.
Naturwissenschaften. 2017 Oct 23;104(11-12):94. doi: 10.1007/s00114-017-1515-z.
Aldehyde oxidases (AOXs) are a family of metabolic enzymes that oxidize aldehydes into carboxylic acids; therefore, they play critical roles in detoxification and degradation of chemicals. By using transcriptomic and genomic approaches, we successfully identified six putative AOX genes (HarmAOX1-6) from cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae). In silico expression profile, reverse transcription (RT)-PCR, and quantitative PCR (qPCR) analyses showed that HarmAOX1 is highly expressed in adult antennae, tarsi, and larval mouthparts, so they may play an important role in degrading plant-derived compounds. HarmAOX2 is highly and specifically expressed in adult antennae, suggesting a candidate pheromone-degrading enzyme (PDE) to inactivate the sex pheromone components (Z)-11-hexadecenal and (Z)-9-hexadecenal. RNA sequencing data further demonstrated that a number of host plants they feed on could significantly upregulate the expression levels of HarmAOX1 in larvae. This study improves our understanding of insect aldehyde oxidases and insect-plant interactions.
醛氧化酶（AOXs）是一类代谢酶，可将醛氧化为羧酸；因此，它们在化学物质的解毒和降解中发挥着关键作用。通过使用转录组学和基因组学方法，我们成功地从棉铃虫（Helicoverpa armigera (Hübner)，鳞翅目：夜蛾科）中鉴定出六个假定的AOX基因（HarmAOX1 - 6）。电子表达谱、逆转录（RT）-PCR和定量PCR（qPCR）分析表明，HarmAOX1在成虫触角、跗节和幼虫口器中高表达，因此它们可能在降解植物衍生化合物中发挥重要作用。HarmAOX2在成虫触角中高度特异性表达，表明它是一种候选的信息素降解酶（PDE），可使性信息素成分（Z）-11-十六碳烯醛和（Z）-9-十六碳烯醛失活。RNA测序数据进一步表明，它们取食的许多寄主植物可显著上调幼虫中HarmAOX1的表达水平。本研究增进了我们对昆虫醛氧化酶和昆虫-植物相互作用的理解。
