Department of Graduate Studies and Pharmaceutical Sciences, University of South Florida, 4202 East Fowler Avenue, Tampa, FL, 33620, USA.
Division of Translational Medicine, Center for Education in Nanobioengineering, University of South Florida, 4202 East Fowler Avenue, Tampa, FL, USA.
Environ Sci Pollut Res Int. 2018 Jan;25(1):704-711. doi: 10.1007/s11356-017-0078-z. Epub 2017 Oct 23.
Algae biomass-fed wastewaters are a promising source of lipid and bioenergy manufacture, revealing substantial end-product investment returns. However, wastewaters would contain lytic pathogens carrying drug resistance detrimental to algae yield and environmental safety. This study was conducted to simultaneously decipher through high-throughput advanced Illumina 16S ribosomal RNA (rRNA) gene sequencing, the cultivable and uncultivable bacterial community profile found in a single sample that was directly recovered from the local wastewater systems. Samples were collected from two previously documented sources including anaerobically digested (AD) municipal wastewater and swine wastewater with algae namely Chlorella spp. in addition to control samples, swine wastewater, and municipal wastewater without algae. Results indicated the presence of a significant level of Bacteria in all samples with an average of approximately 95.49% followed by Archaea 2.34%, in local wastewaters designed for algae cultivation. Taxonomic genus identification indicated the presence of Calothrix, Pseudomonas, and Clostridium as the most prevalent strains in both local municipal and swine wastewater samples containing algae with an average of 17.37, 12.19, and 7.84%, respectively. Interestingly, swine wastewater without algae displayed the lowest level of Pseudomonas strains < 0.1%. The abundance of some Pseudomonas species in wastewaters containing algae indicates potential coexistence between these strains and algae microenvironment, suggesting further investigations. This finding was particularly relevant for the earlier documented adverse effects of some nosocomial Pseudomonas strains on algae growth and their multidrug resistance potential, requiring the development of targeted bioremediation with regard to the beneficial flora.
藻类生物质废水是脂质和生物能源制造的有前途的来源，具有可观的最终产品投资回报。然而，废水中可能含有带有抗药性的裂解病原体，这对藻类产量和环境安全不利。本研究旨在通过高通量先进的 Illumina 16S 核糖体 RNA(rRNA)基因测序，同时解析从当地废水系统直接回收的单个样本中可培养和不可培养细菌群落的特征。样本取自两个先前记录的来源，包括厌氧消化(AD)城市废水和含有藻类的猪废水，此外还有对照样本、猪废水和不含藻类的城市废水。结果表明，所有样本中都存在大量的细菌，平均约为 95.49%，其次是古菌 2.34%，这些样本都是为藻类培养而设计的当地废水。分类属的鉴定表明，在含有藻类的当地城市和猪废水中，Calothrix、Pseudomonas 和 Clostridium 是最常见的菌株，平均分别为 17.37%、12.19%和 7.84%。有趣的是，不含藻类的猪废水显示出最低水平的假单胞菌菌株 <0.1%。废水中某些假单胞菌属的丰度表明这些菌株与藻类微环境之间存在潜在的共存关系，这表明需要进一步研究。这一发现对于先前记录的某些医院获得性假单胞菌菌株对藻类生长及其多药耐药潜力的不利影响尤为重要，需要针对有益菌群开发有针对性的生物修复。
