Department of Microbiology, Pusan National University, Busan 609-735, Republic of Korea.
Bioresour Technol. 2013 Mar;131:515-20. doi: 10.1016/j.biortech.2012.12.176. Epub 2013 Jan 5.
In order to reduce input cost for microalgal cultivation, we investigated the feasibility of wastewater taken from a municipal WWTP in Busan, Korea as wastewater nutrients. The wastewaters used in this study were the effluent from a primary settling tank (PS), the effluent from an anaerobic digestion tank (AD), the conflux of wastewaters rejected from sludge-concentrate tanks and dewatering facilities (CR), and two combined wastewaters of AD:PS (10:90, v/v) and AD:CR (10:90, v/v). Chlorella sp. ADE5, which was isolated from the AD, was selected for the feasibility test. The highest biomass production (3.01 g-dry cell weight per liter) of the isolate was obtained with the combined wastewater ADCR, and it was 1.72 times higher than that with BG 11 medium. Interestingly, the cells cultivated with wastewater containing PS wastewater were easily separated from the culture and improved lipid content, especially oleic acid content, in their cells.
为了降低微藻培养的投入成本,我们研究了从韩国釜山的一个城市污水处理厂(WWTP)中提取废水作为废水营养物的可行性。本研究中使用的废水分别为:初沉池(PS)出水、厌氧消化池(AD)出水、浓缩池和脱水设施中剩余污泥废水的混合液(CR)、以及两种 AD:PS(10:90,v/v)和 AD:CR(10:90,v/v)的组合废水。从 AD 中分离出的绿球藻 ADE5 被选作可行性测试的菌株。该分离株在 ADCR 组合废水中的生物量最高(每升干细胞重量 3.01 克),比 BG 11 培养基高 1.72 倍。有趣的是,用含有 PS 废水的废水培养的细胞很容易从培养液中分离出来,并且提高了细胞中的脂质含量,特别是油酸含量。