Stander Maria A, Van Wyk Ben-Erik, Taylor Malcolm J C, Long Helen S
Department of Botany and Plant Biotechnology, University of Johannesburg , P.O. Box 524, Auckland Park, Johannesburg, 2006 South Africa.
J Agric Food Chem. 2017 Nov 29;65(47):10270-10281. doi: 10.1021/acs.jafc.7b03942. Epub 2017 Nov 13.
Tea samples from 17 populations of "wild tea" ecotypes Aspalathus linearis (rooibos tea) and 2 populations of Aspalathus pendula were analyzed. Recent advances in column technology together with high-resolution mass spectrometry were applied to improve resolution, facilitating the identification of several new compounds as well as grouping of the wild tea ecotypes according to their chemical composition. The collisional cross-section data obtained from ion mobility-mass spectrometry is reported for the flavonoids in rooibos for the first time. Enzyme pathways for the synthesis of the unique flavonoids found in rooibos tea are also proposed. A. linearis and A. pendula produce similar combinations of main phenolic compounds, with no diagnostically different discontinuities between populations or species. Northern resprouters (Gifberg and Nieuwoudtville) contain higher phenylpropenoic acid glucoside levels while teas from Wupperthal and surrounding areas were found to contain unique dihydrochalcones (phloridzin and a sieboldin analog), which are reported here for the first time.
对来自17个“野生茶”生态型的线叶金雀花（路易波士茶）样本和2个垂枝金雀花种群的茶叶样本进行了分析。采用柱技术的最新进展以及高分辨率质谱法来提高分辨率，这有助于鉴定几种新化合物，并根据其化学成分对野生茶生态型进行分类。首次报道了从离子淌度-质谱法获得的路易波士茶中黄酮类化合物的碰撞截面数据。还提出了路易波士茶中发现的独特黄酮类化合物的合成酶途径。线叶金雀花和垂枝金雀花产生相似的主要酚类化合物组合，种群或物种之间没有诊断上不同的间断。北方再生植株（吉夫贝格和新乌德维尔）含有较高水平的苯丙烯酸葡萄糖苷，而发现来自伍珀塔尔及其周边地区的茶叶含有独特的二氢查耳酮（根皮苷和一种西伯苷类似物），本文首次报道了这两种物质。
