Experimental Eye Research Institute, University Eye Hospital, Ruhr-University Bochum, In der Schornau 23-25, 44892, Bochum, Germany.
Cell Tissue Res. 2018 Feb;371(2):237-249. doi: 10.1007/s00441-017-2710-0. Epub 2017 Oct 24.
Previous studies have revealed a loss of retinal ganglion cells (RGCs) and optic nerve fibers after immunization with the S100B protein. Addition of heat shock protein 27 (HSP27) also leads to a decrease of RGCs. Our present aim has been to analyze various retinal cell types after immunization with S100B or S100B + HSP27 (S100 + HSP). After 28 days, retinas were processed for immunohistology and Western blot. RGCs, immunostained for NeuN, were significantly decreased in the S100 and the S100 + HSP groups. Significantly fewer ChAT cells were noted in both groups, whereas parvalbumin cells were only affected in the S100 + HSP group. Western blot results also revealed fewer ChAT signals in both immunized groups. No changes were noted with regard to PKCα rod bipolar cells, whereas a significant loss of recoverin cone bipolar cells was observed in both groups via immunohistology and Western blot. The presynaptic marker Bassoon and the postsynaptic marker PSD95 were significantly reduced in the S100 + HSP group. Opsin and rhodopsin photoreceptors revealed no changes in either group. Thus, the inner retinal layers are affected by immunization. However, the combination of S100 and HSP27 has a stronger additive effect on the retinal synapses and AII amacrine cells.
先前的研究表明，免疫 S100B 蛋白会导致视网膜神经节细胞 (RGCs) 和视神经纤维丢失。添加热休克蛋白 27 (HSP27) 也会导致 RGCs 减少。我们目前的目的是分析免疫 S100B 或 S100B+HSP27 (S100+HSP) 后各种视网膜细胞类型。28 天后，对视网膜进行免疫组织化学和 Western blot 处理。用 NeuN 免疫染色的 RGCs 在 S100 和 S100+HSP 组中明显减少。两组 ChAT 细胞均显著减少，而只有 S100+HSP 组的 parvalbumin 细胞受到影响。Western blot 结果还显示，两组免疫后的 ChAT 信号均减少。PKCα 杆状双极细胞没有变化，而通过免疫组织化学和 Western blot 观察到两组 recoverin 锥体双极细胞明显丢失。突触前标记物 Bassoon 和突触后标记物 PSD95 在 S100+HSP 组中显著减少。视蛋白和视紫红质光感受器在任何一组中均无变化。因此，内视网膜层受到免疫的影响。然而，S100 和 HSP27 的组合对视网膜突触和 AII 无长突细胞有更强的附加作用。
