Milovancev Milan, Townsend Katy L, Bracha Shay, Gorman Elena, Curran Kaitlin, Russell Duncan S
Department of Clinical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, Oregon.
Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, Oregon.
Vet Surg. 2018 Jan;47(1):36-43. doi: 10.1111/vsu.12731. Epub 2017 Oct 24.
Quantify changes in the circumferential lengths of surgical margins of resected canine mast cell tumors (MCT) and soft tissue sarcomas (STS) between the time of collection and histopathology.
Prospective, hypothesis-driven, clinical study.
Two hundred and thirty-seven margins from 69 excised tumors (50 MCT and 19 STS) in 51 client-owned dogs.
The lengths of surgical margins were recorded (eg, cranial, caudal, dorsal, and ventral) for each tumor at 5 time points: intraoperatively (in vivo), immediately after excision (ex vivo), after formalin fixation (postfixation), once mounted on glass slides (subgross), and as histologically tumor-free margins (HTFMs).
Compared to in vivo dimensions, the length of surgical margins at each processing step (ie, ex vivo, postfixation, subgross, and HTFM) was reduced by a median of 3.0, 5.0, 6.0, and 8.8 mm for MCT; 2.5, 2.0, 5.0, and 5.0 mm for STS. All processing steps resulted in significant reductions among MCT samples (P < .0001), except between postfixation vs subgross, and for STS samples (P < .0001), except between ex vivo vs postfixation and subgross vs HTFM. The maximum reduction in the total length of margins (from in vivo to HTFM) was 29.6 and 24.2 mm for MCT and STS, respectively.
Surgical margin length reductions occur due to a combination of physical factors (eg, tissue elasticity, myofibril contraction, and histologic processing) and biological factors (eg, microscopic tumor infiltration into the grossly normal surgical margin).
These data provide information relevant to evidence-based surgical planning and may influence patient morbidity in the most commonly encountered cutaneous malignancies of dogs.
量化切除的犬肥大细胞瘤（MCT）和软组织肉瘤（STS）手术切缘在采集时与组织病理学检查时圆周长度的变化。
前瞻性、假设驱动的临床研究。
51只客户拥有的犬身上69个切除肿瘤（50个MCT和19个STS）的237个切缘。
在5个时间点记录每个肿瘤手术切缘的长度（如头侧、尾侧、背侧和腹侧）：术中（体内）、切除后立即（离体）、福尔马林固定后（固定后）、一旦安装在载玻片上（亚大体）以及作为组织学上无肿瘤切缘（HTFM）。
与体内尺寸相比，MCT在每个处理步骤（即离体、固定后、亚大体和HTFM）的手术切缘长度分别减少了中位数3.0、5.0、6.0和8.8毫米；STS分别减少了2.5、2.0、5.0和5.0毫米。除了固定后与亚大体之间以及STS样本（P <.0001）中除离体与固定后以及亚大体与HTFM之间外，所有处理步骤在MCT样本中均导致显著减少（P <.0001）。MCT和STS切缘总长度（从体内到HTFM）的最大减少分别为29.6和24.2毫米。
手术切缘长度减少是由于物理因素（如组织弹性、肌原纤维收缩和组织学处理）和生物学因素（如微观肿瘤浸润到大体正常的手术切缘）共同作用的结果。
这些数据为基于证据的手术规划提供了相关信息，并可能影响犬最常见皮肤恶性肿瘤患者术后的发病率。
