College of Chemistry and Chemical Engineering, Central South University, Changsha, China.
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, China.
J Sep Sci. 2017 Dec;40(24):4780-4787. doi: 10.1002/jssc.201700333. Epub 2017 Nov 20.
A screening method using α-amylase-functionalized magnetic graphene oxide combined with high-speed counter-current chromatography was proposed and utilized to screen and separate α-amylase inhibitors from extract of Solanum nigrum. The α-amylase-functionalized magnetic graphene oxide was characterized and found to demonstrate satisfactory structure, magnetic response (24.5 emu/g), and reusability (retained 90% of initial activity after five cycles). The conditions for the screening with α-amylase functionalized magnetic graphene oxide were optimized and set at pH 7.0 and 25°C. As a result, two potent flavonoid compounds, apigenin-7-O-glucuronide (1) and astragalin (2), were separated and collected through high-speed counter-current chromatography and subjected to high-performance liquid chromatography analysis with purity higher than 90% (according to HPLC data), which were identified as α-amylase inhibitors. These results suggested that utilization of α-amylase functionalized magnetic graphene oxide in the rapid screening and isolation bioactive compounds from complex natural products is a feasible and environmentally friendly method.
一种使用α-淀粉酶功能化磁性氧化石墨烯结合高速逆流色谱的筛选方法被提出并用于从龙葵提取物中筛选和分离α-淀粉酶抑制剂。α-淀粉酶功能化磁性氧化石墨烯进行了表征，结果表明其具有良好的结构、磁响应（24.5 emu/g）和可重复使用性（经过五次循环后保留了初始活性的 90%）。优化了使用α-淀粉酶功能化磁性氧化石墨烯进行筛选的条件，设定为 pH 7.0 和 25°C。结果，通过高速逆流色谱分离和收集了两种有效的黄酮类化合物，芹菜素-7-O-葡萄糖醛酸苷（1）和毛蕊花糖苷（2），并通过高效液相色谱分析，纯度均高于 90%（根据 HPLC 数据），被鉴定为α-淀粉酶抑制剂。这些结果表明，利用α-淀粉酶功能化磁性氧化石墨烯从复杂天然产物中快速筛选和分离生物活性化合物是一种可行且环保的方法。
