维生素 E 涂层透析器抑制氧化应激。
Vitamin E-Coated Dialyzer Inhibits Oxidative Stress.
机构信息
Department of Healthcare and Regulatory Sciences, School of Pharmaceuticy, Showa University, Shinagawa-ku, Japan.
出版信息
Blood Purif. 2017;44(4):288-293. doi: 10.1159/000478971. Epub 2017 Oct 25.
AIM
To examine the effects of vitamin E-coated dialyzer on oxidative stress in vitro.
METHODS
A dialyzer with a synthetic polymer membrane (APS-11SA) and vitamin E-coated dialyzer (VPS-11SA) were connected to a blood tubing line, and U937 cells were circulated in the device. The circulating fluid was collected at 1, 2, 5, 10, 25, and 50 cycles, which are estimated numbers of passes through the dialyzer. Intracellular reactive oxygen species (ROS) production, malondialdehyde (MDA), and Cu/Zn-superoxide dismutase (SOD) were quantified.
RESULTS
Intracellular ROS production was increased in the first cycle by APS-11SA and was decreased throughout the experiment by VPS-11SA. Intracellular ROS production in the VPS-11SA device was lower, and MDA levels were decreased. MDA levels were lower during VPS-11SA processing than during APS-11SA processing. Cu/Zn-SOD levels remained unchanged.
CONCLUSION
Our results highlight anti-oxidative-stress effects of a vitamin E-coated dialyzer.
目的
研究维生素 E 包被透析器对体外氧化应激的影响。
方法
将具有合成聚合物膜（APS-11SA）和维生素 E 包被透析器（VPS-11SA）的透析器与血液管路连接，并使 U937 细胞在装置中循环。在 1、2、5、10、25 和 50 个循环（估计通过透析器的次数）时收集循环液。定量测定细胞内活性氧（ROS）产生、丙二醛（MDA）和铜/锌-超氧化物歧化酶（SOD）。
结果
APS-11SA 在第一个循环中增加了细胞内 ROS 的产生，而 VPS-11SA 在整个实验过程中降低了 ROS 的产生。VPS-11SA 装置中的细胞内 ROS 产生较低，MDA 水平降低。VPS-11SA 处理期间的 MDA 水平低于 APS-11SA 处理期间的 MDA 水平。Cu/Zn-SOD 水平保持不变。
结论
我们的结果强调了维生素 E 包被透析器的抗氧化应激作用。
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