Santarelli Xavier, Cabanne Charlotte
Bordeaux INP, CBMN, UMR 5248, F-33600, Pessac, France.
Curr Protein Pept Sci. 2019;20(1):14-21. doi: 10.2174/1389203718666171024121137.
Mixed mode chromatography offers a diversity of ligands, each providing a new selectivity. This allows the design of novel purification processes with reduced column steps. Structure of ligands is based on both hydrophobic and ionic groups. Thanks to its salt tolerance, crude extracts or post-IEX samples can be loaded directly without conditioning. The selectivity could be enhanced by modulating elution parameters or by using additives. More importantly, mixed mode chromatography could be as effective as affinity chromatography for mAb purification processes. Mixed mode chromatography opens the way to short and economical processes.
混合模式色谱提供了多种配体，每种配体都具有新的选择性。这使得能够设计出减少柱步骤的新型纯化工艺。配体的结构基于疏水基团和离子基团。由于其耐盐性，粗提物或离子交换色谱后的样品无需预处理即可直接上样。通过调节洗脱参数或使用添加剂可以提高选择性。更重要的是，在单克隆抗体纯化过程中，混合模式色谱的效果可与亲和色谱相媲美。混合模式色谱为简短且经济的工艺开辟了道路。
