Halan Vivek, Maity Sunit, Bhambure Rahul, Rathore Anurag S
Zumutor Biologics Private Limited, Yeshwanthpur, Bangalore, India.
National Chemical Laboratory, Pune, India.
Curr Protein Pept Sci. 2019;20(1):4-13. doi: 10.2174/1389203718666171020103559.
Process chromatography forms the core of purification of biotherapeutics. The unparalleled selectivity that it offers over other alternatives combined with the considerable robustness and scalability make it the unit operation of choice in downstream processing. It is typical to have three to five chromatography steps in a purification process for a biotherapeutic. Generally, these steps offer different modes of separation such as ion-exchange, reversed phase, size exclusion, and hydrophobic interaction. In the past decade, multimodal chromatography has emerged as an alternative to the traditional modes. It involves use of more than one mode of separation and typically combines ion-exchange and hydrophobic interactions to achieve selectivity and sensitivity. Over the last decade, numerous authors have demonstrated the significant potential that multimode chromatography offers as a protein purification tool. This review aims to present key recent developments that have occurred on this topic together with a perspective on future applications of multimodal chromatography.
制备色谱法是生物治疗药物纯化的核心。与其他方法相比，它具有无与伦比的选择性，同时具备相当高的稳定性和可扩展性，这使其成为下游加工中首选的单元操作。在生物治疗药物的纯化过程中，通常会有三到五个色谱步骤。一般来说，这些步骤提供不同的分离模式，如离子交换、反相、尺寸排阻和疏水相互作用。在过去十年中，多模式色谱法已成为传统模式的替代方法。它涉及使用多种分离模式，通常结合离子交换和疏水相互作用来实现选择性和灵敏度。在过去十年中，众多作者已经证明了多模式色谱法作为蛋白质纯化工具的巨大潜力。本综述旨在介绍该主题最近的关键进展以及对多模式色谱法未来应用的展望。
