同一基因型的猪肺炎支原体在一个封闭猪群中持续存在至少两年。
Persistence of the same genetic type of Mycoplasma hyopneumoniae in a closed herd for at least two years.
作者信息
Rebaque Florencia, Camacho Pablo, Parada Julián, Lucchesi Paula, Ambrogi Arnaldo, Tamiozzo Pablo
机构信息
Departamento de Patología Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, Río Cuarto, Córdoba CP 5800, Argentina.
Departamento de Patología Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, Río Cuarto, Córdoba CP 5800, Argentina; CONICET, Argentina.
出版信息
Rev Argent Microbiol. 2018 Apr-Jun;50(2):147-150. doi: 10.1016/j.ram.2017.05.002. Epub 2017 Oct 20.
Two cross-sectional studies were carried out in 2013 and 2015 monitoring for Mycoplasma hyopneumoniae presence in a swine farm. In these studies, the genetic diversity of M. hyopneumoniae was assessed in clinical specimens using a Multiple Locus Variable-number tandem repeat Analysis (MLVA) targeting P97 R1, P146 R3 and H4 loci. The samples from August 2015 showed the MLVA profile prevalent in June 2013, therefore it can be concluded that a same genetic type of M. hyopneumoniae can persist for at least two years in a closed herd. In addition, the nested PCR reactions implemented in this study showed to be useful for MLVA typing in non-invasive clinical samples.
2013年和2015年开展了两项横断面研究，监测一个猪场中猪肺炎支原体的存在情况。在这些研究中，使用针对P97 R1、P146 R3和H4位点的多位点可变数目串联重复序列分析（MLVA）对临床样本中猪肺炎支原体的遗传多样性进行了评估。2015年8月的样本显示出2013年6月流行的MLVA图谱，因此可以得出结论，同一遗传类型的猪肺炎支原体可以在一个封闭猪群中持续存在至少两年。此外，本研究中实施的巢式PCR反应显示对非侵入性临床样本的MLVA分型有用。
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