Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota 55108, USA (Betlach, Sponheim, Pieters); Swine Vet Center, St. Peter, Minnesota 56082, USA (Betlach, Dalquist); Boehringer Ingelheim Animal Health USA Inc., Duluth, Georgia 30096, USA (Fano, Sponheim); Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota 55108, USA (Valeris-Chacin, Singer).
Can J Vet Res. 2020 Oct;84(4):310-313.
The aim of this study was to assess the genetic variability of within various swine production flows. Four positive production flows, composed of 4 production stages, were selected for this study. Laryngeal and/or bronchial swabs were collected from each production stage within a flow, for a period of 4 months up to 3 years. A multiple-locus variable-number tandem repeat analysis was performed to assess the genetic variation of within and across production flows through the identification of variable-number tandem repeat (VNTR) types. A maximum of 6 VNTR types were identified in a single flow, in which VNTR types appeared to be flow specific. An identical VNTR type was detected across several production stages for up to 3 years. In this study, minimal genetic variation was evidenced within and across production flows.
本研究旨在评估 在不同猪生产流程中的遗传变异。为此，选择了四个 阳性生产流程，每个流程包含四个生产阶段。在 4 个月至 3 年的时间内，从每个流程的每个生产阶段采集喉和/或支气管拭子。通过识别数量可变串联重复（VNTR）类型，进行多位点可变数量串联重复分析，以评估 在生产流程内和跨生产流程中的遗传变异。在单个流程中最多可鉴定出 6 种 VNTR 类型，这些 VNTR 类型似乎是流程特异性的。多达 3 年内，在几个生产阶段检测到相同的 VNTR 类型。在本研究中，在生产流程内和跨生产流程中都没有明显的遗传变异。
