Ashcheulova Darya O, Efimova Lina V, Lushchyk Aliaksandr Ya, Yantsevich Aliaksei V, Baikov Alexander N, Pershina Alexandra G
Siberian State Medical University, 2, Moskovsky trakt, 634050 Tomsk, Russia; National Research Tomsk Polytechnic University, 30, Lenina Ave., Tomsk 634050, Russia.
Siberian State Medical University, 2, Moskovsky trakt, 634050 Tomsk, Russia.
Protein Expr Purif. 2018 Mar;143:38-44. doi: 10.1016/j.pep.2017.10.011. Epub 2017 Oct 21.
Radiolabeled peptides derived from ubiquicidine (UBI) are of great interest for early and highly accurate scintigraphic detection and differentiation of infection and sterile inflammation. In the present work the recombinant antimicrobial peptide UBI - a fragment of the human natural cationic peptide ubiquicidine - was produced in Escherichia coli for the first time. The insoluble expression of the peptide in fusion with ketosteroid isomerase provided high yield, about 6 mg of UBI per liter. We developed an approach to produce the antimicrobial peptide UBI, that encompasses inclusion body isolation and size exclusion chromatography. This method could be the basis for industrial biotechnological production of diagnostic system components that are in high demand.
源自泛喹杀菌肽（UBI）的放射性标记肽对于感染和无菌性炎症的早期及高度准确的闪烁显像检测与鉴别具有重大意义。在本研究中，重组抗菌肽UBI（人类天然阳离子肽泛喹杀菌肽的一个片段）首次在大肠杆菌中产生。该肽与酮类固醇异构酶融合时的不溶性表达实现了高产率，每升约产生6毫克UBI。我们开发了一种生产抗菌肽UBI的方法，包括包涵体分离和尺寸排阻色谱法。该方法可为高需求的诊断系统组件的工业生物技术生产奠定基础。
