Zivancevic-Simonovic Snezana, Mihaljevic Olgica, Kostic Irena, Ilic Nevenka, Mihajlovic Dusan, Vasiljevic Dragan, Mijatovic-Teodorovic Ljiljana, Miletic-Drakulic Svetlana, Colic Miodrag
Department of Pathophysiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia
Department of Pathophysiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
Ann Clin Lab Sci. 2017 Sep;47(5):541-545.
Published data indicate the involvement of eosinophil granulocytes and eosinophil cationic protein (ECP) in tumor defense. The aim of this study was to analyze serum ECP concentrations in patients with differentiated thyroid cancer (DTC) before, 3 days and 7 days after radioactive iodine (131-I) therapy. Association of ECP concentrations with histological type of tumor, stage of disease and/or levels of selected T-helper 2 (Th2) cytokines was examined. The study population included 17 DTC patients and 10 control subjects. ECP was measured by fluoroimmunoassay (FIA). Th2 (cytokines interleukin 4 (IL-4), interleukin 5 (IL-5), and interleukin 13 (IL-13)) were determined by enzyme-linked immunosorbent assays (ELISA). We found that ECP values in DTC patients before radioactive iodine therapy were approximately two-fold higher than in the controls, but the difference was statistically significant only if the patients with DTC and associated Hashimoto thyroiditis (HT) were included. There was no correlation between the serum concentrations of IL-5 and ECP. Radioactive iodine therapy led to a decrease in serum ECP level which did not follow the decline in serum protein levels. Additional studies are needed to determine the significance of these findings.
已发表的数据表明嗜酸性粒细胞和嗜酸性粒细胞阳离子蛋白（ECP）参与肿瘤防御。本研究的目的是分析分化型甲状腺癌（DTC）患者在放射性碘（131-I）治疗前、治疗后3天和7天的血清ECP浓度。研究了ECP浓度与肿瘤组织学类型、疾病分期和/或选定的辅助性T细胞2（Th2）细胞因子水平之间的关联。研究人群包括17例DTC患者和10例对照受试者。通过荧光免疫测定法（FIA）测量ECP。通过酶联免疫吸附测定法（ELISA）测定Th2（细胞因子白细胞介素4（IL-4）、白细胞介素5（IL-5）和白细胞介素13（IL-13））。我们发现，放射性碘治疗前DTC患者的ECP值比对照组高约两倍，但只有纳入患有DTC和相关桥本甲状腺炎（HT）的患者时，差异才有统计学意义。IL-5血清浓度与ECP之间无相关性。放射性碘治疗导致血清ECP水平下降，且这一下降与血清蛋白水平的下降不一致。需要进一步研究以确定这些发现的意义。
