Ali Mahesheema, Rajapakshe Deepthi, Cao Liyun, Devaraj Sridevi
Department of Pathology & Immunology, Baylor College of Medicine, Houston, TX USA.
Texas Children's Hospital, Houston, TX USA.
Ann Clin Lab Sci. 2017 Sep;47(5):638-640.
Recent studies have reported that biotin interferes with certain immunoassays. In this study, we evaluated the analytical interference of biotin on immunoassays that use streptavidin-biotin in our pediatric hospital. We tested the effect of different concentrations of biotin (1.5-200 ng/ml) on TSH, Prolactin, Ferritin, CK-MB, β-hCG, Troponin I, LH, FSH, Cortisol, Anti-HAV antibody (IgG and IgM), assays on Ortho Clinical Diagnostic Vitros 5600 Analyzer. Biotin (up to 200 ng/mL) did not significantly affect Troponin I and HAV assays. Biotin (up to 12.5 ng/ml) resulted in <10% bias in CK-MB, β-hCG, AFP, Cortisol, Ferritin assays and biotin >6.25 ng/mL significantly affected TSH (>20% bias) assay. Prolactin was significantly affected even at low levels (Biotin 1.5 ng/mL). Thus, we recommend educating physicians about biotin interference in common immunoassays and adding an electronic disclaimer.
近期研究报告称生物素会干扰某些免疫测定。在本研究中，我们在我院儿科评估了生物素对使用链霉亲和素 - 生物素的免疫测定的分析干扰。我们在奥森多临床诊断公司的Vitros 5600分析仪上测试了不同浓度生物素（1.5 - 200 ng/ml）对促甲状腺激素（TSH）、催乳素、铁蛋白、肌酸激酶同工酶（CK - MB）、β - 人绒毛膜促性腺激素（β - hCG）、肌钙蛋白I、促黄体生成素（LH）、促卵泡生成素（FSH）、皮质醇、抗甲型肝炎病毒抗体（IgG和IgM）检测的影响。生物素（高达200 ng/mL）对肌钙蛋白I和甲型肝炎病毒检测无显著影响。生物素（高达12.5 ng/ml）导致肌酸激酶同工酶、β - hCG、甲胎蛋白（AFP）、皮质醇、铁蛋白检测的偏差<10%，而生物素>6.25 ng/mL显著影响促甲状腺激素检测（偏差>20%）。即使在低水平（生物素1.5 ng/mL）时，催乳素也受到显著影响。因此，我们建议告知医生生物素对常见免疫测定的干扰，并添加电子免责声明。
