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苏云金芽孢杆菌(H-14)和球形芽孢杆菌对加利福尼亚州积水型蚊子的防治效果及田间评估

Efficacy and field evaluation of Bacillus thuringiensis (H-14) and B. sphaericus against floodwater mosquitoes in California.

作者信息

Mulla M S, Darwazeh H A, Ede L, Kennedy B, Dulmage H T

机构信息

Department of Entomology, University of California, Riverside 92521.

出版信息

J Am Mosq Control Assoc. 1985 Sep;1(3):310-5.

PMID:2906673
Abstract

The microbial control agents Bacillus thuringiensis (H-14) and B. sphaericus were evaluated in laboratory and field against Psorophora columbiae. Bacillus sphaericus strain 2362 was also tested in the field against Aedes melanimon. Psorophora columbiae was slightly more susceptible than Culex quinquefasciatus to active strains of B. sphaericus. The LC90 for active strains ranged from 0.013 to 0.069 mg/liter. In field trials, aqueous suspensions of primary powder of B. sphaericus 2362 and 1593 yielded 98-99% reduction in larvae at the rates of 0.1 to 0.25 lb/acre of the primary powder. Granular formulations of Bt (H-14) were evaluated against Ps. columbiae, yielding 96-99% control of larvae at rates ranging from 1 to 10 lb/acre of the granules, depending on the potency and type of the formulations. Aedes melanimon was slightly less susceptible than Ps. columbiae to B. sphaericus 2362. In warmer water a rate of 0.25 lb/acre of the primary powder yielded 88% control, while this same rate in cool weather yielded only 4% reduction. A rate of 0.5 lb/acre of the primary powder was needed to obtain 94% control of larvae in cool weather.

摘要

在实验室和田间对微生物防治剂苏云金芽孢杆菌(H - 14)和球形芽孢杆菌进行了针对哥伦比亚骚蚊的评估。球形芽孢杆菌菌株2362也在田间针对黑胸伊蚊进行了测试。哥伦比亚骚蚊对球形芽孢杆菌的活性菌株比致倦库蚊略更敏感。活性菌株的LC90范围为0.013至0.069毫克/升。在田间试验中,球形芽孢杆菌2362和1593原粉的水悬浮液以0.1至0.25磅/英亩的原粉用量使幼虫数量减少了98 - 99%。苏云金芽孢杆菌(H - 14)的颗粒制剂针对哥伦比亚骚蚊进行了评估,根据制剂的效力和类型,以1至10磅/英亩的颗粒用量可实现对幼虫96 - 99%的防治效果。黑胸伊蚊对球形芽孢杆菌2362的敏感性略低于哥伦比亚骚蚊。在较温暖的水中,0.25磅/英亩的原粉用量可实现88%的防治效果,而在凉爽天气下相同用量仅使幼虫数量减少4%。在凉爽天气下需要0.5磅/英亩的原粉用量才能实现对幼虫94%的防治效果。

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引用本文的文献

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J Med Entomol. 2010 May;47(3):345-8. doi: 10.1603/me09241.
2
The mitogen-activated protein kinase p38 is involved in insect defense against Cry toxins from Bacillus thuringiensis.丝裂原活化蛋白激酶 p38 参与昆虫对苏云金芽孢杆菌 Cry 毒素的防御。
Insect Biochem Mol Biol. 2010 Jan;40(1):58-63. doi: 10.1016/j.ibmb.2009.12.010. Epub 2009 Dec 28.