Baruah I, Das S C
Defence Research Laboratory, Tezpur, Assam, India.
J Commun Dis. 1994 Jun;26(2):82-7.
Laboratory and field trials were carried out with two formulations of Bacillus thuringiensis and four strains of Bacillus sphaericus (B 42, B 64, B 87 and B 33) against mosquito larvae in different breeding habitats of Tezpur, Assam. LC90 of B. thuringiensis var israelensis (formulation Teknar) against Ae. albopictus, Cx. quinquefasciatus and Cx. gelidus were recorded as 0.443, 0.453 and 2.15 ppm respectively and LC90 of B. thuringiensis (Deltox: VCRC B-17) against Ae. albopictus, Cx. quinquefasciatus, Cx. gelidus and Cx. malayi were 8.414, 11.22, 5.24 and 6.761 ppm respectively. LC90 of B. sphaericus strains B 42, B 64, B 87 and B 33 against Cx. quinquefasciatus were 0.055, 0.115, 0.046 and 0.257 ppm respectively. At the dosage of 1 l/ha 87 per cent mortality was achieved after 24 hrs with Bti and it increased to 90-95 per cent at 1.5 l/ha. In polluted cemented drains 93-97 per cent kill of Cx. quinquefasciatus was observed at 2.5 l/ha. Out of four strains of B. sphaericus evaluated, strain B 87 was found to be the most effective as 87-96 per cent kill was achieved with only 0.1 kg/ha for Cx. quinquefasciatus, Cx. vishnui and A. vagus. For others 0.2 kg/ha dosage, eliminated 96-100 per cent Cx. vishnui gr. for B 42, 92-93 per cent for B 64 and 90-93 per cent for B 33 strain.
在阿萨姆邦特兹布尔不同的蚊虫滋生地，使用两种苏云金芽孢杆菌制剂和四株球形芽孢杆菌（B 42、B 64、B 87和B 33）进行了实验室和现场试验。以色列亚种苏云金芽孢杆菌（制剂Teknar）对白纹伊蚊、致倦库蚊和吉氏库蚊的LC90分别记录为0.443、0.453和2.15 ppm，苏云金芽孢杆菌（Deltox：VCRC B - 17）对白纹伊蚊、致倦库蚊、吉氏库蚊和马来库蚊的LC90分别为8.414、11.22、5.24和6.761 ppm。球形芽孢杆菌菌株B 42、B 64、B 87和B 33对致倦库蚊的LC90分别为0.055、0.115、0.046和0.257 ppm。使用苏云金芽孢杆菌以色列亚种以1升/公顷的剂量处理后，24小时内死亡率达到87%，在1.5升/公顷时死亡率增至90 - 95%。在污染的水泥排水渠中，以2.5升/公顷的剂量处理时，观察到致倦库蚊的杀灭率为93 - 97%。在所评估的四株球形芽孢杆菌中，菌株B 87被发现是最有效的，对于致倦库蚊、维氏库蚊和迷走按蚊，仅用0.1千克/公顷就能达到87 - 96%的杀灭率。对于其他蚊虫，0.2千克/公顷的剂量，B 42菌株对维氏库蚊种群的杀灭率为96 - 100%，B 64菌株为92 - 93%，B 33菌株为90 - 93%。
